Weng Lin, Smits Patrick, Wauters Jan, Merregaert Jozef
Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium.
Genomics. 2002 Jul;80(1):62-70. doi: 10.1006/geno.2002.6806.
CHODL, a novel human gene encoding chondrolectin, was isolated by PCR screening. It is localized at chromosome 21q21 and consists of six exons and five introns. The open reading frame of CHODL encodes a type I transmembrane protein containing a single carbohydrate recognition domain (CRD) of C-type lectins in its extracellular portion. CHODL was detected as a 2.6-kb transcript by northern blot using enriched human testis RNA. RT-PCR analysis revealed preferential expression of CHODL in testis, prostate, and spleen. Immunohistochemistry demonstrated that the expression of CHODL is mainly limited to vascular muscle of testis, smooth muscle of prostate stroma, heart muscle, skeletal muscle, crypts of small intestine, and red pulp of spleen. Western blot analysis revealed that CHODL is an N-glycosylated protein with a molecular weight of approximately 36 kDa. In transiently transfected COS1 cells, CHODL shows a predominantly perinuclear localization. Although the predicted CHODL protein shares a significant homology (45% overall and 60% within the CRD) with layilin, a recently identified hyaluronan receptor, we failed to detect a specific interaction between CHODL and hyaluronan using cetylpyridinium chloride precipitation.
通过聚合酶链反应（PCR）筛选分离出了一种编码软骨凝集素的新型人类基因CHODL。它定位于21号染色体q21，由六个外显子和五个内含子组成。CHODL的开放阅读框编码一种I型跨膜蛋白，其胞外部分含有一个C型凝集素的单一碳水化合物识别结构域（CRD）。使用富集的人类睾丸RNA，通过Northern印迹检测到CHODL为2.6 kb的转录本。逆转录-聚合酶链反应（RT-PCR）分析显示CHODL在睾丸、前列腺和脾脏中优先表达。免疫组织化学表明，CHODL的表达主要局限于睾丸的血管平滑肌、前列腺基质的平滑肌、心肌、骨骼肌、小肠隐窝和脾脏红髓。蛋白质免疫印迹分析显示CHODL是一种N-糖基化蛋白，分子量约为36 kDa。在瞬时转染的COS1细胞中，CHODL主要定位于核周。尽管预测的CHODL蛋白与最近鉴定的透明质酸受体层粘连蛋白具有显著的同源性（总体为45%，CRD内为60%），但我们使用十六烷基吡啶氯化物沉淀法未能检测到CHODL与透明质酸之间的特异性相互作用。
