肿瘤细胞上DcR3的表达与对FasL敏感性之间的相关性。

Correlation between expression of DcR3 on tumor cells and sensitivity to FasL.

作者信息

Li Wenzhu, Zhang Changgong, Chen Caixia, Zhuang Guohong

机构信息

Anti-Cancer Research Center, Medical College, Xiamen University, 422 Siming South Road, Xiamen, Fujian 361005, China.

出版信息

Cell Mol Immunol. 2007 Dec;4(6):455-60.

PMID:18163957

Abstract

To investigate the correlation between sensitivity to Fas ligand (FasL) and expression level of decoy receptor 3 (DcR3) on tumor cell surface, Fas/DcR3 mRNA expression was detected by RT-PCR. Anti-DcR3 mAb was used to detect expression level of DcR3 on surface of tumor cells by flow cytometry. Caspase-8, caspase-9, caspase-3, Bcl-2 expressions were analyzed by Western blot, respectively. Sensitivity to apoptosis induced by FasL was determined by Annexin V apoptosis kit. The expressions of DcR3 on the surface of tumor cells from high to low were approximately 35.3% in BGC823 cells, and 21.6% in MCF-7 cells, respectively. The apoptotic rates induced by FasL from low to high were 15.6% in BGC823 cells, and 58.2% in MCF-7 cells, respectively. There was a significant correlation between the expression levels of DcR3 with FasL-inducing apoptosis.

摘要

为研究肿瘤细胞表面对Fas配体（FasL）的敏感性与诱饵受体3（DcR3）表达水平之间的相关性，采用逆转录聚合酶链反应（RT-PCR）检测Fas/DcR3 mRNA表达。应用抗DcR3单克隆抗体（mAb）通过流式细胞术检测肿瘤细胞表面DcR3的表达水平。分别采用蛋白质免疫印迹法分析半胱天冬酶-8（Caspase-8）、半胱天冬酶-9（Caspase-9）、半胱天冬酶-3（Caspase-3）、Bcl-2的表达情况。采用膜联蛋白V凋亡检测试剂盒测定FasL诱导的细胞凋亡敏感性。肿瘤细胞表面DcR3的表达水平由高到低分别为：BGC823细胞约为35.3%，MCF-7细胞约为21.6%。FasL诱导的凋亡率由低到高分别为：BGC823细胞为15.6%，MCF-7细胞为58.2%。DcR3表达水平与FasL诱导的细胞凋亡之间存在显著相关性。