Guo Lijin, Huang Weiling, Tong Feng, Chen Xiaolan, Cao Sen, Xu Haiping, Luo Wei, Li Zhenhui, Nie Qinghua
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China.
Front Physiol. 2020 Oct 30;11:573676. doi: 10.3389/fphys.2020.573676. eCollection 2020.
Coccidiosis is a major hazard to the chicken industry, but the host's immune response to coccidiosis remains unclear. Here, we performed coccidia challenge in 28-day-old ROSS 308 broilers and selected the bursa from the three most severely affected individuals and three healthy individuals for RNA sequencing. We obtained 347 DEGs from RNA-seq and found that 7 upregulated DEGs were enriched in pathway. As the DEGs with the highest expression abundance in these 7 genes, was speculated to participate in the process of host's immune response to coccidiosis. It is showed that can polarize macrophages to M1 subtype and promote inflammatory cytokines expression. In addition, the expression of suppressed HD11 cells apoptosis by downregulating Fas signal pathway. Besides, -mediated macrophages immunity activation can be reversed by apoptosis. Overall, our study indicates that upregulated in BAE, is capable of aggravating the inflammatory response by inhibiting macrophages apoptosis via downregulating Fas signal pathway, which may participate in host's immune response to coccidiosis.
球虫病是养鸡业的主要危害，但宿主对球虫病的免疫反应仍不清楚。在此，我们对28日龄的ROSS 308肉鸡进行球虫攻击，并从三个受影响最严重的个体和三个健康个体中选取法氏囊进行RNA测序。我们从RNA测序中获得了347个差异表达基因（DEGs），并发现7个上调的DEGs在 途径中富集。作为这7个基因中表达丰度最高的DEGs，推测其参与宿主对球虫病的免疫反应过程。结果表明， 可将巨噬细胞极化为M1亚型并促进炎性细胞因子表达。此外， 的表达通过下调Fas信号通路抑制HD11细胞凋亡。此外， 介导的巨噬细胞免疫激活可被凋亡逆转。总体而言，我们的研究表明，在BAE中上调的 能够通过下调Fas信号通路抑制巨噬细胞凋亡来加重炎症反应，这可能参与宿主对球虫病的免疫反应。
