Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host's Immune Response to Coccidiosis.

Coccidiosis is a major hazard to the chicken industry, but the host's immune response to coccidiosis remains unclear. Here, we performed coccidia challenge in 28-day-old ROSS 308 broilers and selected the bursa from the three most severely affected individuals and three healthy individuals for RNA sequencing. We obtained 347 DEGs from RNA-seq and found that 7 upregulated DEGs were enriched in pathway. As the DEGs with the highest expression abundance in these 7 genes, was speculated to participate in the process of host's immune response to coccidiosis. It is showed that can polarize macrophages to M1 subtype and promote inflammatory cytokines expression. In addition, the expression of suppressed HD11 cells apoptosis by downregulating Fas signal pathway. Besides, -mediated macrophages immunity activation can be reversed by apoptosis. Overall, our study indicates that upregulated in BAE, is capable of aggravating the inflammatory response by inhibiting macrophages apoptosis via downregulating Fas signal pathway, which may participate in host's immune response to coccidiosis.