Uno Yasuhiro, Suzuki Yutaka, Wakaguri Hiroyuki, Sakamoto Yoshiko, Sano Hitomi, Osada Naoki, Hashimoto Katsuyuki, Sugano Sumio, Inoue Ituro
Division of Genetic Diagnosis, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan.
FEBS Lett. 2008 Jan 23;582(2):351-8. doi: 10.1016/j.febslet.2007.12.031. Epub 2007 Dec 31.
The liver, a major organ for drug metabolism, is physiologically similar between monkeys and humans. However, the paucity of identified genes has hampered a deep understanding of drug metabolism in monkeys. To provide such a genetic resource, 28655 expressed sequence tags (ESTs) were generated from a cynomolgus monkey liver full-length enriched cDNA library, which contained 23 unique ESTs homologous to human drug-metabolizing enzymes. Our comparative genomics approach identified nine lineage-specific candidate ESTs, including three drug-metabolizing enzymes, which could be important for understanding the physiological differences between monkeys and humans.
肝脏是药物代谢的主要器官，在生理上猴子和人类相似。然而，已鉴定基因的匮乏阻碍了对猴子药物代谢的深入理解。为了提供这样一种遗传资源，从食蟹猴肝脏全长富集cDNA文库中生成了28655个表达序列标签（EST），该文库包含23个与人类药物代谢酶同源的独特EST。我们的比较基因组学方法鉴定出9个谱系特异性候选EST，包括三种药物代谢酶，这对于理解猴子和人类之间的生理差异可能很重要。
