Osada Naoki, Hirata Makoto, Tanuma Reiko, Suzuki Yutaka, Sugano Sumio, Terao Keiji, Kusuda Jun, Kameoka Yosuke, Hashimoto Katsuyuki, Takahashi Ichiro
Department of Biomedical Resources, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
BMC Res Notes. 2009 Sep 29;2:199. doi: 10.1186/1756-0500-2-199.
Consolidating transcriptome data of non-human primates is essential to annotate primate genome sequences, and will facilitate research using non-human primates in the genomic era. Macaca fascicularis is a macaque monkey that is commonly used for biomedical and ecological research.
We constructed cDNA libraries of Macaca fascicularis, derived from tissues obtained from bone marrow, liver, pancreas, spleen, and thymus of a young male, and kidney of a young female. In total, 5'-end sequences of 56,856 clones were determined. Including the previously established cDNA libraries from brain and testis, we have isolated 112,587 cDNAs of Macaca fascicularis, which correspond to 56% of the curated human reference genes.
These sequences were deposited in the public sequence database as well as in-house macaque genome database http://genebank.nibio.go.jp/qfbase/. These data will become valuable resources for identifying functional parts of the genome of macaque monkeys in future studies.
整合非人类灵长类动物的转录组数据对于注释灵长类动物基因组序列至关重要，并且将促进在基因组时代使用非人类灵长类动物进行研究。食蟹猕猴是一种常用于生物医学和生态学研究的猕猴。
我们构建了食蟹猕猴的cDNA文库，这些文库来源于一只年轻雄性的骨髓、肝脏、胰腺、脾脏和胸腺以及一只年轻雌性的肾脏组织。总共测定了56,856个克隆的5'端序列。包括先前建立的来自大脑和睾丸的cDNA文库，我们已经分离出112,587个食蟹猕猴的cDNA，这相当于经过整理的人类参考基因的56%。
这些序列已存入公共序列数据库以及内部猕猴基因组数据库http://genebank.nibio.go.jp/qfbase/。这些数据将成为未来研究中鉴定猕猴基因组功能部分的宝贵资源。
