Kuji Naoaki, Yoshii Tsuyoshi, Hamatani Toshio, Hanabusa Hideji, Yoshimura Yasunori, Kato Shingo
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Fertil Steril. 2008 Nov;90(5):1983-7. doi: 10.1016/j.fertnstert.2007.09.025. Epub 2007 Dec 31.
To compare buoyant density and sedimentation kinetics of human immunodeficiency virus 1 (HIV-1) in two sperm-washing media, Percoll and Pureception.
Laboratory study.
University hospital.
PATIENT(S): None.
INTERVENTION(S): Buoyant density and sedimentation kinetics of HIV-1 particles (MOLT-4/LAI strain) were measured in Percoll and Pureception using isopyknic ultracentrifugation and continuous-density-gradient centrifugation.
MAIN OUTCOME MEASURE(S): The HIV-1 particles were detected and semiquantified using a reverse transcription polymerase chain reaction (RT-PCR) for HIV-1 RNA.
RESULT(S): Calculated buoyant density of HIV-1 was approximately 1.042 in both media in isopyknic centrifugation. However, most HIV-1 particles were found in fractions with specific gravity less than 1.04 in both media, even after 40 minutes of density-gradient centrifugation at 1,600 g. Small viral accumulations were observed at the bottom of the tube in Pureception density gradients.
CONCLUSION(S): Although we found very high efficiency of HIV-1 removal using density-gradient centrifugation, a minute quantity of virus was found at the bottom of the gradient tube when Pureception was used as the medium.
比较人类免疫缺陷病毒1型（HIV-1）在两种精子洗涤介质（Percoll和Pureception）中的浮力密度和沉降动力学。
实验室研究。
大学医院。
无。
使用等密度超速离心和连续密度梯度离心法，在Percoll和Pureception中测量HIV-1颗粒（MOLT-4/LAI株）的浮力密度和沉降动力学。
使用针对HIV-1 RNA的逆转录聚合酶链反应（RT-PCR）检测并半定量HIV-1颗粒。
在等密度离心中，两种介质中HIV-1的计算浮力密度约为1.042。然而，即使在1600 g下进行40分钟的密度梯度离心后，两种介质中大多数HIV-1颗粒仍存在于比重小于1.04的组分中。在Pureception密度梯度中，在管底部观察到少量病毒聚集。
尽管我们发现使用密度梯度离心法去除HIV-1的效率非常高，但当使用Pureception作为介质时，在梯度管底部发现了微量病毒。
