用于从劣质精液中筛选活动精子的不连续Percoll梯度制备法的效率

Efficiency of discontinuous Percoll gradients preparation for selection of motile sperm from poor quality semens.

作者信息

Sukcharoen N

机构信息

Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

出版信息

J Med Assoc Thai. 1994 Dec;77(12):633-8.

PMID:7759973

Abstract

Efficiency of discontinuous Percoll gradients preparation for selection of motile sperm from poor quality semens was studied in 106 semen samples, of which 52 were normozoospermia, 9 were oligozoospermia, 29 were asthenozoospermia, and 16 were oligoasthenozoospermia. Sperms from each semen sample were recovered by the discontinuous two-layer (50%/100%) Percoll centrifugation technique. Overall motile sperm recovery rate was about 27 per cent. The results obtained in this study indicated that sperm selection by this method from oligozoospermic and oligoasthenozoospermic semen samples was not as good as normozoospermic semen samples because per cent progressive motility showed no statistically significant improvement after sperm selection in oligozoospermic and oligoasthenozoospermic semen samples. Moreover, total sperm recovery rate and motile sperm recovery rate of oligozoospermic semen samples were less than normozoospermic samples significantly (p < 0.01 and p < 0.005, respectively).

摘要

在106份精液样本中研究了采用不连续Percoll梯度法从质量较差的精液中筛选活动精子的效率，其中52份为正常精子症，9份为少精子症，29份为弱精子症，16份为少弱精子症。通过不连续两层（50%/100%）Percoll离心技术回收每份精液样本中的精子。活动精子总体回收率约为27%。本研究结果表明，用该方法从少精子症和少弱精子症精液样本中筛选精子不如从正常精子症精液样本中筛选效果好，因为在少精子症和少弱精子症精液样本中筛选精子后，进行性活动率没有统计学上的显著提高。此外，少精子症精液样本的总精子回收率和活动精子回收率明显低于正常精子症样本（分别为p<0.01和p<0.005）。