美沙酮在双灌注早产人胎盘小叶中的转运。
Transfer of methadone across the dually perfused preterm human placental lobule.
作者信息
Nanovskaya Tatiana N, Nekhayeva Ilona A, Hankins Gary D V, Ahmed Mahmoud S
机构信息
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX 77555-0587, USA.
出版信息
Am J Obstet Gynecol. 2008 Jan;198(1):126.e1-4. doi: 10.1016/j.ajog.2007.06.073.
OBJECTIVE
The objective of the study was to determine the effect of gestational age and P-glycoprotein expression on transplacental transfer of methadone.
STUDY DESIGN
Dual perfusion of placental lobule was utilized. Methadone (200 ng/mL) and its [3H]-isotope were cotransfused from the maternal-to-fetal circuit with the marker compound antipyrine (20 microg/mL) and its [14C]-isotope. Concentration of the drugs in trophoblast tissue and both circuits was determined by liquid scintillation spectrometry.
RESULTS
Fetal transfer rate of methadone in preterm placentas was 19 +/- 5.8%, and in term placentas it was 31 +/- 9.7% (P < .01). Clearance index of methadone in preterm placentas (0.57 +/- 0.2) was lower than in term placentas (0.95 +/- 0.3, P < .01). P-glycoprotein expression was higher in preterm than term placentas.
CONCLUSION
The ex vivo transfer of methadone across preterm placentas is 30% lower than in term placentas.
目的
本研究的目的是确定胎龄和P-糖蛋白表达对美沙酮经胎盘转运的影响。
研究设计
采用胎盘小叶双灌注法。将美沙酮（200 ng/mL）及其[3H]同位素与标记化合物安替比林（20 μg/mL）及其[14C]同位素从母血循环共输注至胎儿血循环。通过液体闪烁光谱法测定滋养层组织和两个循环中药物的浓度。
结果
早产胎盘中美沙酮的胎儿转运率为19±5.8%，足月胎盘为31±9.7%（P<.01）。早产胎盘中美沙酮的清除指数（0.57±0.2）低于足月胎盘（0.95±0.3，P<.01）。早产胎盘的P-糖蛋白表达高于足月胎盘。
结论
美沙酮经早产胎盘的体外转运比足月胎盘低30%。
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