Honchar M V, Korpan Ia I, Sibirnyĭ A A
Ukr Biokhim Zh (1978). 1991 Nov-Dec;63(6):63-7.
The photometric method is suggested for ethanol analysis by means of purified alcohol oxidase isolated from a wild strain of methylotrophic yeast Hansenula polymorpha. Three different chromogenic systems for peroxidative oxidation together with alcohol oxidase reaction have been used. For the first time the intact cells of the catalase-negative mutant as a source of alcohol oxidase are employed in the photometric alcohol assay. The prospects for application of the latter method for practical purposes are discussed.
建议采用光度法，通过从多形汉逊酵母野生菌株中分离出的纯化乙醇氧化酶来分析乙醇。已使用了三种不同的用于过氧化物氧化以及乙醇氧化酶反应的显色系统。在光度法乙醇测定中首次采用过氧化氢酶阴性突变体的完整细胞作为乙醇氧化酶的来源。讨论了后一种方法实际应用的前景。
