肾上腺皮质腺苷酸环化酶。钙离子是否参与促肾上腺皮质激素的刺激作用？
Adrenal cortex adenylate cyclase. Is Ca2+ involved in ACTH stimulation?
作者信息
Glossmann H, Gips H
出版信息
Naunyn Schmiedebergs Arch Pharmacol. 1976;292(2):199-203. doi: 10.1007/BF00498593.
PMID:181684
Abstract
The chelator EGTA inhibits the activation of bovine adrenal cortex adenylate cyclase by ACTH. Hormonal response is restored by addition of Ca2+, Mn2+ and other cations which are able to significantly reduce the concentration of uncomplexed EGTA in the adenylate cyclase assy medium. Time course studies reveal that the enzyme in the activated state (induced by adenylate cyclase reagents and hormone or by pretreatment with hormone) is resistant to EGTA inhibition.
摘要
螯合剂乙二醇双四乙酸（EGTA）可抑制促肾上腺皮质激素（ACTH）对牛肾上腺皮质腺苷酸环化酶的激活作用。通过添加钙离子（Ca2+）、锰离子（Mn2+）和其他阳离子可恢复激素反应，这些阳离子能够显著降低腺苷酸环化酶测定培养基中未络合的EGTA浓度。时间进程研究表明，处于激活状态的酶（由腺苷酸环化酶试剂和激素诱导或经激素预处理诱导）对EGTA抑制具有抗性。
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