巨噬细胞移动抑制因子和细胞周期蛋白D1的表达与肝细胞癌肿瘤大小及转移的相关性

[Correlations of expressions of macrophage migration inhibitor factor and cyclin D1 with tumor size and metastasis of hepatocellular carcinoma].

作者信息

Xia Jin-Tang, Li Wen, Liu Yun-Jian, Chen Lian-Zhou, Wu Zhao-Feng, Zhang Long-Juan, Wang Qian

机构信息

Department of Hepatobiliary Surgery, First Municipal Hospital of Guangzhou, Guangzhou Medical College, Guangzhou, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2007 Dec;15(12):918-21.

PMID:18171527

Abstract

OBJECTIVE

To explore the possible relationship between the expressions of macrophage migration inhibitor factor (MIF), cyclin D1, cyclin-dependent kinase 4 (CDK4), phosphorylated-retinoblastoma susceptibility gene product Rb protein (phospho-Rb) and the development of hepatocellular carcinoma (HCC).

METHODS

93 HCC tissues and 5 normal liver tissues were used to investigate the expressions of MIF, cyclin D1, CDK4 and phospho-Rb by tissue microarray and immunohistochemistry methods.

RESULTS

The expression rates of MIF, cyclin D1, CDK4 and phospho-Rb in the HCC tissues were 71%, 41%, 82% and 14% respectively, and in the normal liver tissues, they were 0%, 0%, 80% and 20% respectively. The expression rates of MIF and cyclin D1 were significantly different between the tumor and the normal liver tissues and the expression rates of CDK4 and phospho-Rb were not significantly different between the tumor and the normal liver tissues. The rate difference (69% versus 48%) of MIF expression between the larger tumors (> 3.5 cm) and the smaller tumors (< 3.5 cm) was of statistical significance (P < 0.01). The expression rate (62%) of cyclin D1 in the tumors with metastasis was significantly higher than the expression rate (35%) in the tumors without metastasis (P < 0.05). MIF expression was positively correlated with cyclin D1 expression in the tumor tissues (P < 0.01). CDK4 and phospho-Rb expressions were not significantly associated with the tumor sizes and metastasis status.

CONCLUSION

Our results indicate that MIF and cyclin D1 might be related to the growth and metastasis of HCC.

摘要

目的

探讨巨噬细胞移动抑制因子（MIF）、细胞周期蛋白D1、细胞周期蛋白依赖性激酶4（CDK4）、磷酸化视网膜母细胞瘤易感基因产物Rb蛋白（磷酸化Rb）的表达与肝细胞癌（HCC）发生发展的可能关系。

方法

采用组织芯片和免疫组化方法，检测93例HCC组织和5例正常肝组织中MIF、细胞周期蛋白D1、CDK4和磷酸化Rb的表达。

结果

HCC组织中MIF、细胞周期蛋白D1、CDK4和磷酸化Rb的表达率分别为71%、41%、82%和14%，正常肝组织中分别为0%、0%、80%和20%。肿瘤组织与正常肝组织中MIF和细胞周期蛋白D1的表达率差异有统计学意义，而CDK4和磷酸化Rb的表达率差异无统计学意义。较大肿瘤（>3.5 cm）与较小肿瘤（<3.5 cm）之间MIF表达的率差（69%对48%）有统计学意义（P<0.01）。有转移肿瘤中细胞周期蛋白D1的表达率（62%）显著高于无转移肿瘤中的表达率（35%）（P<0.05）。肿瘤组织中MIF表达与细胞周期蛋白D1表达呈正相关（P<0.01）。CDK4和磷酸化Rb的表达与肿瘤大小和转移状态无明显相关性。