Tsuboi I, Morimoto K, Sakuma A, Horie T
First Department of Internal Medicine, Faculty of Medicine, Nihon University.
J Radiat Res. 1991 Dec;32(4):395-403. doi: 10.1269/jrr.32.395.
The effects of irradiation on the growth regulatory function of marrow stromal cells (MSC), and on the committed granulocyte-macrophage progenitor cells (GM-CFC), were investigated using a liquid culture system. CSF activity in the supernatant of irradiated MSC (0-900 rads) increased markedly with the increase of MSC irradiation dose. CSF-inhibitory activity in the supernatant of irradiated MSC (0-900 rads) also increased with the increase of MSC irradiation dose. Furthermore, the activity of exogenous CSF added to the supernatant of 900 rad-irradiated MSC was lost more slowly than that of non-irradiated MSC. These data suggest that irradiation affected CSF production, inhibitor production and consumption of CSF by MSC.
采用液体培养系统研究了辐射对骨髓基质细胞（MSC）生长调节功能以及对定向粒细胞-巨噬细胞祖细胞（GM-CFC）的影响。照射后的MSC（0 - 900拉德）上清液中的集落刺激因子（CSF）活性随MSC照射剂量的增加而显著升高。照射后的MSC（0 - 900拉德）上清液中的CSF抑制活性也随MSC照射剂量的增加而升高。此外，添加到900拉德照射的MSC上清液中的外源性CSF的活性比未照射的MSC的活性丧失得更慢。这些数据表明，辐射影响了CSF的产生、抑制剂的产生以及MSC对CSF的消耗。
