Fedorocko P, Domonkosová A, Kundrátová T, Macková N O, Brezáni P, Fedorocková A
Department of Cellular and Molecular Biology, Faculty of Sciences, Safárik University, Moyzesova, Kosice, Slovak Republic.
Physiol Res. 1996;45(2):93-100.
The effects of single subcutaneous injection of cadmium chloride on haemopoiesis in normal (non-irradiated) or irradiated mice were investigated. Cadmium doses used ranged from 1-8 mg/kg body weight Twenty-four hours after treatment with cadmium (doses from 3 to 8 mg/kg) there were no significant changes in bone marrow cellularity and the granulocyte-macrophage progenitor cell (GM-CFC) number per femur in non-irradiated female ICR mice. Similarly, during the 30-day postinjection period bone marrow cellularity and marrow GM-CFC number in mice treated with a cadmium dose of 5 mg/kg were not significantly different from the control values. Cadmium significantly reduced the lethal effects of gamma rays. In addition, increasing the doses of cadmium administered 24 h prior to sublethal irradiation increased the number of endogenous haemopoietic stem cells (endoCFU-S) in a concentration-dependent manner. Pretreatment with cadmium also decreased the radiation damage to endoCFU-S and haemopoietic progenitor cells committed to granulocyte/macrophage development (GM-CFC). The survival of stem cells was higher and the regeneration of cellularity and GM-CFC of irradiated bone marrow was accelerated in mice pretreated with 5 mg Cd/kg body weight in comparison with saline-injected mice.
研究了单次皮下注射氯化镉对正常（未受辐照）或受辐照小鼠造血功能的影响。所用镉剂量范围为1-8毫克/千克体重。在用镉处理（剂量为3至8毫克/千克）24小时后，未受辐照的雌性ICR小鼠的骨髓细胞密度和每根股骨的粒细胞-巨噬细胞祖细胞（GM-CFC）数量没有显著变化。同样，在注射后30天内，用5毫克/千克镉剂量处理的小鼠的骨髓细胞密度和骨髓GM-CFC数量与对照值没有显著差异。镉显著降低了γ射线的致死效应。此外，在亚致死性辐照前24小时增加镉的给药剂量，以内源性造血干细胞（endoCFU-S）数量呈浓度依赖性增加。镉预处理还减少了对endoCFU-S和定向于粒细胞/巨噬细胞发育的造血祖细胞（GM-CFC）的辐射损伤。与注射生理盐水的小鼠相比，用5毫克镉/千克体重预处理的小鼠干细胞存活率更高，辐照骨髓的细胞密度和GM-CFC的再生加速。
