Rafferty Mark, Walker Carol, Husband David, Helliwell Timothy, Fenton John, Jones Andrew
Head and Neck Oncology Group, Department of Medicine, University of Liverpool, Liverpool, UK.
Eur Arch Otorhinolaryngol. 2008 Jul;265 Suppl 1:S83-7. doi: 10.1007/s00405-007-0558-8. Epub 2008 Jan 3.
The retinoblastoma gene (Rb) is postulated to be important in carcinoma of the larynx. Its cellular protein (pRb) is involved in regulation of the cell cycle and may be influential in the cells response to irradiation injury. From the University of Liverpool Head and Neck Database we identified 35 patients with a T2 N0 laryngeal squamous carcinoma whom received primary irradiation and had a minimum of 5 years follow up. Laser capture microdissection was performed on paired normal and tumour biopsy material to analyse for loss of heterozygosity (LOH) and microsatellite instability (MI) of the Rb gene and immunohistochemistry (IHC) was carried out to detect pRb expression. Of 35 tumours, 13 were normal, 12 had MI and 5 had LOH of the Rb gene. Abnormalities at the Rb locus did not correlate with loss of pRb expression. There was also no significant difference between the distribution of normal and abnormal gene sequences and whether or not the primary laryngeal tumour recurred after radiotherapy. Rb gene abnormalities occurred in one third of T2 N0 laryngeal carcinomas. These were not in isolation predictive of cure by radiotherapy.
视网膜母细胞瘤基因（Rb）被认为在喉癌中具有重要作用。其细胞蛋白（pRb）参与细胞周期调控，可能对细胞对辐射损伤的反应有影响。我们从利物浦大学头颈数据库中识别出35例T2 N0期喉鳞状细胞癌患者，这些患者接受了初次放疗且至少随访5年。对配对的正常和肿瘤活检材料进行激光捕获显微切割，以分析Rb基因的杂合性缺失（LOH）和微卫星不稳定性（MI），并进行免疫组织化学（IHC）检测pRb表达。在35个肿瘤中，13个正常，12个有MI，5个有Rb基因的LOH。Rb基因座的异常与pRb表达缺失无关。正常和异常基因序列的分布与喉原发性肿瘤放疗后是否复发之间也没有显著差异。Rb基因异常发生在三分之一的T2 N0期喉癌中。这些异常并非放疗治愈的独立预测因素。
