Alişkan Hikmet, Colakoğlu Sule, Turunç Tuba, Demiroğlu Yusuf Ziya, Yazic Ayşe Canan, Arslan Hande
Başkent Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Ankara.
Mikrobiyol Bul. 2007 Oct;41(4):591-5.
Although standard tube agglutination (STA) test is the most preferred method for the serodiagnosis of brucellosis, it may yield false negative results because of its incompetence in detecting the blocking (non-agglutinating) antibodies. Blocking antibodies can be detected by Coombs' test (anti-human globulin test), which is generally not performed in laboratories routinely since it is a time consuming and laborious method. Recently more practical Brucellacapt (immunocapture-agglutination) test is being used for the detection of total antibodies without any interference of the presence of blocking ones. The aim of this study was the evaluation of the value of Brucellacapt test in the serodiagnosis of brucellosis. Twenty-five brusellosis patients whose blood cultures yielded Brucella melitensis growth, and 31 healthy subjects as controls, were included to the study. Serum samples obtained from all of the 56 subjects have been searched by STA, Coombs' and Brucellacapt methods, and threshold titre was accepted as 1/160. As a result, 40% (10/25) of culture positive patients were found positive (> or =1/160) by STA, whereas 92% (23/25) were positive (> or =1/160) by both Coombs' and Brucellacapt tests. No positivity was detected in the control group by any one of those three tests. The agreement between Coombs and Brucellacapt methods was found as 100%, while the sensitivity and specificity of Brucellacapt test were estimated as 92% (23/25) and 100% (31/31), respectively, when the culture was accepted as reference method. Although our data have pointed out the high performance of Brucellacapt test in brusellosis serodiagnosis, confirmation with further data carried on extensive and comprehensive study groups, are needed.
尽管标准试管凝集试验（STA）是布鲁氏菌病血清学诊断最常用的方法，但由于其在检测封闭（非凝集）抗体方面能力不足，可能会产生假阴性结果。封闭抗体可通过库姆斯试验（抗人球蛋白试验）检测，由于该方法耗时费力，实验室通常不常规进行。最近，更实用的布鲁氏菌捕获试验（免疫捕获凝集试验）被用于检测总抗体，而不受封闭抗体存在的干扰。本研究的目的是评估布鲁氏菌捕获试验在布鲁氏菌病血清学诊断中的价值。25例血培养分离出羊布鲁氏菌的布鲁氏菌病患者和31名健康对照者纳入研究。对56名受试者的血清样本均采用STA、库姆斯试验和布鲁氏菌捕获试验进行检测，临界滴度设定为1/160。结果，STA检测培养阳性患者中有40%（10/25）呈阳性（≥1/160），而库姆斯试验和布鲁氏菌捕获试验检测阳性率均为92%（23/25）（≥1/160）。对照组三种试验均未检测到阳性。发现库姆斯试验和布鲁氏菌捕获试验方法的一致性为100%，以培养结果为参考方法时，布鲁氏菌捕获试验的敏感性和特异性分别估计为92%（23/25）和100%（31/31）。尽管我们的数据表明布鲁氏菌捕获试验在布鲁氏菌病血清学诊断中具有很高的性能，但仍需要在广泛且全面的研究组中进一步的数据进行验证。
