Ciftçi Cemal, Oztürk Feryal, Oztekin Asil, Karaoğlan Hicran, Saba Rabin, Gültekin Meral, Mamikoğlu Latife
Akdeniz Universitesi Tip Fakültesi, Infeksiyon Hastaliklari ve Klinik Mikrobiyoloji Anabilim Dali, Antalya.
Mikrobiyol Bul. 2005 Jul;39(3):291-9.
Brucellosis which is an important public health problem is a zoonotic disease that causes economic loss, and seen all over the world as well as in our country. The aim of this study was to compare the results of Rose Bengal test, standard tube agglutination test (STA), Coombs tube agglutination test, Rivanol tube agglutination test and ELISA (IgA, IgG and IgM) method, in patients who were suspected to have brucellosis. Blood and serum samples collected from 77 patients were included to the study. Blood cultures were performed from all of the patients and Brucella spp were isolated in 35 (45.4%) of them. At least one of the serological tests for Brucella was found positive in 62 samples (80.5%). When culture positivity accepted as the gold standard, the sensitivities of the tests were calculated as follows; 100% for Rose Bengal, 94.3% for STA, 97.1% for ELISA IgG, 94.3 for ELISA IgA and 71.4 for ELISA IgM. No prezone phenomenon was observed in the samples. Brucella Coombs tube agglutination test did not increase the chance of diagnosis of brucellosis, unlike the results of previous studies. We found that Rose Bengal and STA tests were still efficient methods for brucellosis serodiagnosis. The results of rivanol tube agglutination test were similar to STA test, and this test can be used especially in patients with brucellosis relapse. ELISA test detecting IgA and IgG antibody titers together found to be effective methods increasing the chance of brucellosis diagnosis, and could be helpful for follow up of brucellosis.
布鲁氏菌病是一种重要的公共卫生问题，是一种人畜共患病，会造成经济损失，在世界各地以及我国均有发现。本研究的目的是比较疑似布鲁氏菌病患者中玫瑰红试验、标准试管凝集试验（STA）、库姆斯试管凝集试验、利凡诺试管凝集试验和酶联免疫吸附测定（IgA、IgG和IgM）方法的结果。从77例患者采集的血液和血清样本纳入本研究。对所有患者进行血培养，其中35例（45.4%）分离出布鲁氏菌属。在62份样本（80.5%）中发现至少一种布鲁氏菌血清学检测呈阳性。以培养阳性作为金标准，计算各检测方法的敏感性如下：玫瑰红试验为100%，STA为94.3%，ELISA IgG为97.1%，ELISA IgA为94.3%，ELISA IgM为71.4%。样本中未观察到前带现象。与先前研究结果不同，布鲁氏菌库姆斯试管凝集试验并未增加布鲁氏菌病的诊断机会。我们发现玫瑰红试验和STA试验仍是布鲁氏菌病血清学诊断的有效方法。利凡诺试管凝集试验结果与STA试验相似，该试验尤其可用于布鲁氏菌病复发患者。同时检测IgA和IgG抗体滴度的ELISA试验被发现是增加布鲁氏菌病诊断机会的有效方法，有助于布鲁氏菌病的随访。
