Sutton-Smith P, Beard H, Fazzalari N
Bone and Joint Research Laboratory, Division of Tissue Pathology, Institute of Medical and Veterinary Science and Hanson Institute, Adelaide, SA, Australia.
J Microsc. 2008 Jan;229(Pt 1):60-6. doi: 10.1111/j.1365-2818.2007.01867.x.
Bone quality consists of a number of factors including the amount of bone, bone architecture and the degree of bone mineralization. Quantitative backscattered electron imaging is a technique that allows the degree of mineralization of trabeculae to be assessed and in this study is applied to inter-trochanteric bone biopsies of the proximal femur. Biopsy cores from 22 controls, nine individuals with acute and chronic medical conditions and 22 fragility fracture individuals undergoing total hip replacement were processed into methyl methacrylate, polished and analysed in a Philips XL20 scanning electron microscope. A mean and distribution of weight percent calcium were determined for each individual, and for the control, medically ill and fragility fracture groups. All individuals and groups of individuals showed normal distributions of percent calcium with both the ill and fragility fracture groups being under mineralized relative to the control group. The shape and position of the mineralization distributions suggest that the fragility group resulted from increased bone turnover with a slow progression to under mineralization. In contrast, the ill group appears to have had a more rapid change in the mineralization dynamic. Clear distinctions between the control, fragility fracture and medically ill groups could be seen when the mineralization data were plotted as a scatter graph against age. Graphing the data in this way showed an age-related increase in the degree of mineralization in control individuals with the under-mineralized, fragility fracture group scattered below this normal trend. The medically ill group was similarly less mineralized which highlighted the degree to which medical conditions and treatments can alter bone matrix mineralization.
骨质量由多种因素组成，包括骨量、骨结构和骨矿化程度。定量背散射电子成像技术可用于评估小梁的矿化程度，在本研究中应用于股骨近端转子间骨活检。将来自22名对照者、9名患有急慢性疾病的个体以及22名接受全髋关节置换的脆性骨折个体的活检芯材加工成聚甲基丙烯酸甲酯，进行抛光，并在飞利浦XL20扫描电子显微镜下进行分析。测定了每个个体以及对照组、患病个体组和脆性骨折组的钙重量百分比的平均值和分布情况。所有个体和个体组的钙百分比均呈正态分布，患病个体组和脆性骨折组相对于对照组均矿化不足。矿化分布的形状和位置表明，脆性骨折组是由于骨转换增加并缓慢发展为矿化不足所致。相比之下，患病个体组的矿化动态变化似乎更为迅速。当将矿化数据作为散点图与年龄作图时，可以清楚地看到对照组、脆性骨折组和患病个体组之间的区别。以这种方式绘制数据显示，对照组个体的矿化程度随年龄增长而增加，矿化不足的脆性骨折组散布在该正常趋势之下。患病个体组的矿化程度同样较低，这突出了疾病状况和治疗对骨基质矿化的影响程度。
