Tekin Uğur, Filippi Andreas, Pohl Yango, Kirschner Horst
Department of Oral and Maxillofacial Surgery, School of Dentistry, Bornova-Izmir, Turkey.
Dent Traumatol. 2008 Feb;24(1):38-42. doi: 10.1111/j.1600-9657.2005.00400.x.
A specially composed medium for storing avulsed teeth has been developed. In experimental and clinical studies it could be shown that PDL cells could be kept viable during storage in the medium for up to 53 h. In the present study the medium was tested on pulp cells. A total of 40 immature unerupted third molars with open apices were removed surgically and the teeth were stored in a special cell culture medium (SCCM) or in Hank's balanced salt solution (HBSS) at room temperature for 6, 12, 18 or 24 h. Five teeth were assigned to each group. A total of seven consecutive pulp cross-sections per tooth were examined, resulting in a total of 280 specimens. Viable cells were marked using proliferating cell nuclear antigen (PCNA). The pulp was divided in three regions: apical region (0-0.5 mm), middle region (>0.5-1.5 mm) and coronal region (>1.5 mm). The labelling index (LI) was calculated for the whole cut (regions 1, 2 and 3) and for each region separately. The statistical evaluation was made using the One-way anova and Mann-Whitney Test. Pulp cells of all teeth expressed PCNA. About 110 of 140 specimens in the SCCM and 101 of 140 specimens in the HBSS group showed PCNA-positive cells. The highest LI was observed within the apical region and decreased with increased distance from the medium. No marked cells were observed at a distance of more than 1.5 mm. The LI for both media showed a significant increase with storage intervals (P < 0.05). The pulp cells of teeth stored in SCCM showed a LI nearly twice as high compared to pulp cells of teeth stored in HBSS for the apical and middle region (time interval 6, 18 and 24 h: P < 0.05). The LI for the apical region was found to be 8.43% for the SCCM and 4.50% for the HBSS after 24 h. For the middle region the LI was found to be 2.02% for the SCCM and 0.81% for the HBSS after 24 h. Within the parameters of this study, it appears that the SCCM is able to maintain pulp cell viability better than HBSS. The use of special cell culture media in case of tooth avulsion may be beneficial.
一种专门用于储存脱位牙的培养基已被研发出来。在实验和临床研究中发现，牙周膜细胞在该培养基中储存长达53小时仍可保持存活。在本研究中，对该培养基进行了牙髓细胞测试。通过手术拔除了40颗根尖孔开放的未萌出的未成熟第三磨牙，并将牙齿在室温下分别置于一种特殊细胞培养基（SCCM）或汉克平衡盐溶液（HBSS）中保存6、12、18或24小时。每组分配5颗牙齿。每颗牙齿共检查7个连续的牙髓横断面，总共得到280个标本。使用增殖细胞核抗原（PCNA）标记活细胞。牙髓分为三个区域：根尖区（0 - 0.5毫米）、中区（>0.5 - 1.5毫米）和冠区（>1.5毫米）。计算整个切片（区域1、2和3）以及每个区域单独的标记指数（LI）。采用单因素方差分析和曼 - 惠特尼检验进行统计学评估。所有牙齿的牙髓细胞均表达PCNA。SCCM组140个标本中有约110个、HBSS组140个标本中有101个显示PCNA阳性细胞。根尖区观察到最高的标记指数，且随着与培养基距离的增加而降低。在距离超过1.5毫米处未观察到标记细胞。两种培养基的标记指数均随储存时间显著增加（P < 0.05）。在根尖区和中区，储存于SCCM中的牙齿的牙髓细胞的标记指数几乎是储存于HBSS中的牙齿的牙髓细胞的两倍（时间间隔6、18和24小时：P < 0.05）。24小时后，SCCM组根尖区的标记指数为8.43%，HBSS组为4.50%。24小时后，SCCM组中区的标记指数为2.02%，HBSS组为0.81%。在本研究的参数范围内，似乎SCCM比HBSS更能维持牙髓细胞的活力。牙齿脱位时使用特殊细胞培养基可能是有益的。
