Ozan Fatih, Tepe Bektaş, Polat Zübeyde Akin, Er Kürşat
Department of Oral and Maxillofacial Surgery, School of Dentistry, Cumhuriyet University, Sivas, Turkey.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Feb;105(2):e66-9. doi: 10.1016/j.tripleo.2007.08.002.
The purpose of this study was to determine the ability of the juice of Morus rubra fruit to serve as a temporary storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth.
PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultures were subjected to 4.0%, 2.5%, 1.5%, and 0.5% of the juice of M. rubra fruit, Hank's balanced salt solution (HBSS), phosphate-buffered saline (PBS), and tap water. Tissue culture plates were incubated with experimental media at 37 degrees C for 1, 3, 6, 12, or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was accomplished using 1-way analysis of variance complemented by the Tukey test. The level of significance was 5% (P < .05).
The efficacy of 4.0% and 2.5% M. rubra at 3, 6, and 12 hours was found to be significantly better than HBSS (P < .05). At 24 hours, 4% M. rubra was found to be similar to HBSS, but 2.5% M. rubra was found to be significantly worse than HBSS (P < .005). The results showed that juice of the fruit sample of M. rubra studied at a concentration of 4% was a more effective storage medium than other groups.
Juice of the fruit of M. rubra can be recommended as a suitable transport medium for avulsed teeth.
本研究旨在确定红桑果实汁液作为临时储存介质维持脱位牙牙周膜（PDL）细胞活力的能力。
从健康的第三磨牙获取PDL细胞，并在杜氏改良伊格尔培养基（DMEM）中培养。将培养物分别置于4.0%、2.5%、1.5%和0.5%的红桑果实汁液、汉克平衡盐溶液（HBSS）、磷酸盐缓冲盐水（PBS）和自来水中。将组织培养板与实验培养基在37℃下孵育1、3、6、12或24小时。通过台盼蓝排斥法评估PDL细胞活力。使用单因素方差分析并辅以Tukey检验对数据进行统计分析。显著性水平为5%（P <.05）。
发现3、6和12小时时4.0%和2.5%的红桑果实汁液的效果显著优于HBSS（P <.05）。在24小时时，发现4%的红桑果实汁液与HBSS相似，但2.5%的红桑果实汁液显著差于HBSS（P <.005）。结果表明，所研究的浓度为4%的红桑果实样本汁液是比其他组更有效的储存介质。
红桑果实汁液可推荐作为脱位牙合适的运输介质。
