Havemeister G
Institute for Hygiene and Environmental Medicine, University of Kiel, FRG.
Acta Microbiol Hung. 1991;38(3-4):257-64.
The BRILA-MUG one-tube-test in connection with the MPN-method can be used successfully to determine with sufficient reliability the number of indices of total coliforms and faecal coliforms according to the EC-Directive for bathing waters. Using the one-tube-test and determination of gas production, fluorescence and indole production is, from the hygienic point of view for surface waters, equal in value to subculture and biochemical identification. The test needs only a minimum of material and laboratory staff. Differences between this test and other more extensive tests with several biochemical identification steps are negligible. The work load for bathing water monitoring would not be justified in this case. Due to the occurrence of Aeromonas, the values for total coliforms compared to a determination with biochemical identification are higher in some cases. A high contamination with Aeromonas indicates a high polluted surface water with a high degree of eutrophication. This is an important additional criterion for the evaluation of bathing waters, even when a direct dependence on faecal contamination does not exist in every case. The degree of an up-to-date faecal contamination can be estimated by the pollution with faecal coliforms.
BRILA-MUG单管试验与MPN法相结合，可成功地根据欧盟沐浴水指令，以足够的可靠性确定总大肠菌群和粪大肠菌群的指标数量。从地表水的卫生角度来看，使用单管试验并测定产气、荧光和吲哚产生，在价值上等同于传代培养和生化鉴定。该试验仅需最少的材料和实验室工作人员。此试验与其他具有多个生化鉴定步骤的更广泛试验之间的差异可忽略不计。在这种情况下，沐浴水监测的工作量是不合理的。由于气单胞菌的出现，与生化鉴定测定相比，总大肠菌群的值在某些情况下更高。气单胞菌的高污染表明地表水受到高度污染且富营养化程度高。这是评估沐浴水的一个重要附加标准，即使并非在每种情况下都直接依赖粪便污染。粪便大肠菌群的污染可用于估计近期粪便污染的程度。
