Aleksić S, Bockemühl J, Schulze G, Havemeister G, Heinemeyer E A, Müller H E, von Pritzbuer E
Medizinaluntersuchungsanstalt am Hygienischen Institut Hamburg.
Zentralbl Hyg Umweltmed. 1990 Oct;190(4):395-403.
In order to test the usefulness of BRILA-MUG (= Fluorocult) medium (Merck) for isolation and identification of total coliforms and faecal coliforms in surface water according to the EC guidelines for bathing waters a total of 969 strains of different Enterobacteriaceae and Vibrionaceae species was examined under different culture conditions. These included 486 E. coli (reference strains of O-groups 1-170, enterotoxin and Verotoxin-producing strains), 149 Salmonella (subspecies I-VI), 92 Yersinia, 44 Shigella, 64 Vibrio, 16 Aeromonas, and 118 strains of other Enterobacteriaceae species. After 48 h incubation at 36 degrees C 372 (82.0%) of 486 E. coli showed the typical reactions of gas formation, fluorescence (beta-glucuronidase activity), and indole production. Examination of fluorescence after addition of 1 N NaOH (0.5 ml), or testing of indole production after subculture in tryptophane containing broth improved the amount of typical reactions to 434 (95.4%). Incubation at 44 degrees C for 48 h gave less favourable results as compared with that at 36 degrees C. Out of 483 strains of other species 3.9% Salmonella strains of subspecies II-IV, 6.25% Citrobacter freundii, and 50% Edwardsiella tarda strains yielded reactions typical of E. coli. Shigella and Yersinia strains occasionally produced indole or fluorescence, but never visible gas.
为了根据欧盟沐浴水指导方针，测试BRILA - MUG（= Fluorocult）培养基（默克公司）用于地表水总大肠菌群和粪大肠菌群分离与鉴定的效用，在不同培养条件下对总共969株不同的肠杆菌科和弧菌科菌种进行了检测。这些菌种包括486株大肠杆菌（O群1 - 170参考菌株、产肠毒素和产志贺毒素菌株）、149株沙门氏菌（亚种I - VI）、92株耶尔森菌、44株志贺菌、64株弧菌、16株气单胞菌以及118株其他肠杆菌科菌种。在36℃孵育48小时后，486株大肠杆菌中有372株（82.0%）呈现出产气、荧光（β - 葡萄糖醛酸酶活性）和吲哚产生的典型反应。加入1N氢氧化钠（0.5ml）后检测荧光，或在含色氨酸肉汤中继代培养后检测吲哚产生，可使典型反应的数量增加到434株（95.4%）。与36℃孵育相比，44℃孵育48小时的结果较差。在483株其他菌种中，3.9%的沙门氏菌亚种II - IV菌株、6.25%的弗氏柠檬酸杆菌和50%的迟缓爱德华氏菌菌株呈现出大肠杆菌的典型反应。志贺菌和耶尔森菌菌株偶尔会产生吲哚或荧光，但从未观察到明显的气体产生。
