Wang Fen, Li Zhao-hui
Department of Forensic Medicine, Zhongshan school of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Fa Yi Xue Za Zhi. 2007 Oct;23(5):328-31.
To screen the differentially expressed microRNAs(miRNA) between H2O2-induced PC12 cell apoptosis and normal PC12 cells with microarray chips.
PC12 cells were treated with different concentrations of H2O2 for 12 hours, and then the cell viabilities were evaluated by MTT assay and cell apoptosis rates were assessed by flow cytometry (FCM). miRNAs were isolated from control (0 nmol/L H2O2) and H2O2 treated (at concentrations of 50, 100, 200, and 400 nmol/L H2O2, respectively) groups of the PC12 cells, respectively, and were then detected and analyzed by microarray chips.
The P12 cell viabilities in control and H2O2 treated group were (92 +/- 9.8)%, (90 +/- 14.70)%, (80 +/- 13.85)%, (54 +/- 12.23)%, and (22 +/- 7.35)%, respectively. The apoptosis rates for them were 2.6%, 5.2%, 7.2%, 10.4%, 16.6%, and 72.2% accordingly. Expression of 68 miRNAs and 46 miRNAs were detected in both control group and the H2O2 treated groups. Of the 46 miRNAs in the H2O2 treated groups, 39 were similar to that of the control group and 6 were significantly down-regulated.
Our preliminary data may provide a new potential foundation for further study of pathogenesis and its treatment of nerve cell apoptosis caused by cerebral ischemia reperfusion.
运用基因芯片筛选过氧化氢（H2O2）诱导的PC12细胞凋亡与正常PC12细胞之间差异表达的微小RNA（miRNA）。
用不同浓度的H2O2处理PC12细胞12小时，然后通过MTT法评估细胞活力，通过流式细胞术（FCM）评估细胞凋亡率。分别从PC12细胞的对照组（0 nmol/L H2O2）和H2O2处理组（分别为50、100、200和400 nmol/L H2O2）中分离miRNA，然后用基因芯片进行检测和分析。
对照组和H2O2处理组的PC12细胞活力分别为（92±9.8）%、（90±14.70）%、（80±13.85）%、（54±12.23）%和（22±7.35）%。相应的凋亡率分别为2.6%、5.2%、7.2%、10.4%、16.6%和72.2%。在对照组和H2O2处理组中均检测到68个miRNA和46个miRNA的表达。在H2O2处理组的46个miRNA中，39个与对照组相似，6个显著下调。
我们的初步数据可能为进一步研究脑缺血再灌注所致神经细胞凋亡的发病机制及其治疗提供新的潜在依据。
