D6S1043和D12S391基因座在法医亲子鉴定中的应用

[Application of D6S1043 and D12S391 loci in forensic paternity testing].

作者信息

Guo Hong, Lin Yuan, Liu Yan, Que Ting-zhi, Yan Pin-hua, Zhao Zhen-min, Li Cheng-tao, Li Li

机构信息

Medical School of Soochow University, Suzhou 215123, China.

出版信息

Fa Yi Xue Za Zhi. 2007 Oct;23(5):345-6.

PMID:18175572

Abstract

OBJECTIVE

The aim was to investigate the polymorphisms of D6S1043 and D12S391 loci among Han population and evaluate their values in paternity testing. MERTHODS: By using fluorescence dye-labeled primers and capillary electrophoresis, the allele frequencies of the two STR loci among 192 unrelated individuals were investigated.

RESULTS

Twelve alleles were observed in both D6S1043 and D12S391 loci. The ranges of allele frequencies were from 0.0026 to 0.1719 and from 0.0026 to 0.2292, respectively. The discrimination power of D6S1043 and D12S391 were 0.9656 and 0.9510. The Average exclusion probability in paternity testing for duos were 0.573 and 0.510. The Average exclusion probability in paternity testing for trios were 0.731 and 0.679, respectively. The genotypes frequencies met Hardy-Weinberg equilibrium expectation.

CONCLUSION

The results show that D6S1043 and D12S391 have high values in forensic paternity testing.

摘要

目的

研究汉族人群中D6S1043和D12S391基因座的多态性，并评估其在亲子鉴定中的应用价值。方法：采用荧光染料标记引物和毛细管电泳技术，对192名无关个体的两个STR基因座的等位基因频率进行研究。

结果

在D6S1043和D12S391基因座均观察到12个等位基因。等位基因频率范围分别为0.0026至0.1719和0.0026至0.2292。D6S1043和D12S391的鉴别力分别为0.9656和0.9510。二联体亲子鉴定的平均排除概率分别为0.573和0.510。三联体亲子鉴定的平均排除概率分别为0.731和0.679。基因型频率符合Hardy-Weinberg平衡预期。