液相色谱-串联质谱多反应监测同时筛查血液中的22种有毒生物碱
[Simultaneous screening for 22 poisonous alkaloids in blood by liquid chromatography-tandem mass spectrometry with multiple-reaction monitoring].
作者信息
Liu Wei, Shen Min, Shen Bao-hua, Xiang Ping, Wu He-jian
机构信息
Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
出版信息
Fa Yi Xue Za Zhi. 2007 Oct;23(5):349-52.
OBJECTIVE
To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening for 22 poisonous alkaloids in blood.
METHODS
This method involves a liquid-liquid extraction (LLE) followed by liquid chromatography-tandem mass spectrometry with multi-ple-reaction monitoring (MRM). After blood was extracted with buprenorphine as the internal standard, the target compounds were analyzed with LC-MS/MS-ESI in the positive ionization mode.
RESULTS
Identification was based on the compound's retention time and two precursor-to-product ion transitions. The limits of detection ranged from 0.1 ng/mL to 20 ng/mL in blood.
CONCLUSION
The method was sufficiently selective and sensitive to detect poisonous alkaloids and can be applied in forensic and clinical toxicology.
目的
建立一种液相色谱 - 串联质谱（LC-MS/MS）法，用于同时筛查血液中的22种有毒生物碱。
方法
该方法包括液 - 液萃取（LLE），随后采用多反应监测（MRM）的液相色谱 - 串联质谱法。以丁丙诺啡为内标对血液进行萃取后，采用LC-MS/MS-ESI在正离子模式下分析目标化合物。
结果
根据化合物的保留时间和两个前体离子到产物离子的跃迁进行鉴定。血液中的检测限范围为0.1 ng/mL至20 ng/mL。
结论
该方法对检测有毒生物碱具有足够的选择性和灵敏度，可应用于法医和临床毒理学。
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