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[揉浆法硅藻检测的应用]

[Application of diatom detection using knead pulp method].

作者信息

Ye Guang-hua, Yu Lin-sheng, Zhang Yi-gu

机构信息

Department of Forensic Medicine, Wenzhou Medical College, Wenzhou 325035, China.

出版信息

Fa Yi Xue Za Zhi. 2007 Oct;23(5):355-7.

PMID:18175575
Abstract

OBJECTIVE

A novel technology for detection of diatom was discussed.

METHODS

Five grams of testing sample were taken and the organics were removed using simple mechanical knead pulp method. The homogenized samples were concentrated by centrifugation, smeared, and then examined under light microscope.

RESULTS

Except for a few feather's grains, the vast majority of diatom could be identified easily with clear diatom striations. The organic diatom could also be easily detected by this methodology.

CONCLUSION

The detection of diatom using knead pulp method is not only simple and inexpensive with a higher successful rate, but also causes nearly no harm to human and environment.

摘要

目的

探讨一种检测硅藻的新技术。

方法

取5克测试样品,采用简单的机械揉浆法去除有机物。将匀化后的样品离心浓缩,涂片,然后在光学显微镜下检查。

结果

除少数羽毛状颗粒外,绝大多数硅藻易于识别,硅藻条纹清晰。该方法也能轻松检测出有机硅藻。

结论

采用揉浆法检测硅藻不仅简单、成本低且成功率较高,而且对人和环境几乎无害。

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