Jacobson K B, Arlinghaus H F, Buchanan M V, Chen C H, Glish G L, Hettich R L, McLuckey S A
Biology Division, Atom Sciences, Inc., Oak Ridge, Tennessee.
Genet Anal Tech Appl. 1991 Dec;8(8):223-9. doi: 10.1016/1050-3862(91)90032-m.
The ability of the mass spectrometer to analyze collectively the masses of DNA fragments that are produced in the Sanger procedure for sequencing may allow the gel electrophoresis step to be eliminated. On the other hand, if gel electrophoresis is required, the use of resonance ionization spectroscopy coupled to a mass spectrometer may enable much faster analysis of DNA bands labeled with stable isotopes. Other combinations of labeling of the DNA and its mass spectrometric analysis with or without gel electrophoresis are also considered. Recent advances in these areas of mass spectrometry are reviewed.
质谱仪对桑格测序法中产生的DNA片段的质量进行集体分析的能力，可能使凝胶电泳步骤得以省略。另一方面，如果需要凝胶电泳，将共振电离光谱与质谱仪联用，可能会使对用稳定同位素标记的DNA条带的分析速度大大加快。还考虑了DNA标记及其质谱分析（无论有无凝胶电泳）的其他组合。本文综述了这些质谱领域的最新进展。
