Kuroyanagi Masanori, Ikeda Ryuya, Gao Hui Yuan, Muto Norio, Otaki Keisuke, Sano Toshikazu, Kawahara Nobuo, Nakane Takahisa
Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Hiroshima, Japan.
Chem Pharm Bull (Tokyo). 2008 Jan;56(1):60-3. doi: 10.1248/cpb.56.60.
In the screening of biologically active constituents from woody plants, the methanol extract of leaves of Chamaecyparis obtusa showed potent neurite outgrowth-promoting activity in neuronal PC12 cells. The ethyl acetate-soluble fraction of the methanol extract showed potent activity and was separated by means of various chromatographic methods to give the two new compounds 1 and 2, as well as 11 known lignan and sesquiterpene derivatives. The structures of the new compounds were determined to be 9-O-acetyldihydrosesamin (1) and 9-O-(11-hydroxyeudesman-4-yl)dihydrosesamin (2), respectively, in NMR studies including 2D-NMR experiments. Of the 13 compounds, the known compound hinokinin (5) and the new compound 2 showed potent neurite outgrowth-promoting activity in PC 12 cells.
在对木本植物生物活性成分的筛选中，钝叶扁柏叶的甲醇提取物在神经元PC12细胞中显示出强大的促神经突生长活性。甲醇提取物的乙酸乙酯可溶部分显示出强大活性，并通过各种色谱方法进行分离，得到了两种新化合物1和2，以及11种已知的木脂素和倍半萜衍生物。通过包括二维核磁共振实验在内的核磁共振研究确定，新化合物的结构分别为9 - O - 乙酰基二氢芝麻素（1）和9 - O -（11 - 羟基桉烷 - 4 - 基）二氢芝麻素（2）。在这13种化合物中，已知化合物扁柏脂素（5）和新化合物2在PC12细胞中显示出强大的促神经突生长活性。
