Cachet T, de Turck K, Roets E, Hoogmartens J
Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.
J Pharm Biomed Anal. 1991;9(7):547-55. doi: 10.1016/0731-7085(91)80176-a.
A column-switching technique is described for LC of erythromycin. The method allows, in about 1 h, the separation of erythromycin A from all its known potential impurities, except erythromycin D, which is a minor impurity. The switching technique combines two columns (7.5 cm x 4.6 mm and 25.0 cm x 4.6 mm) both packed with RSil C 18 LL 10 microns. The mobile phase is acetonitrile-tetrabutylammonium sulphate (0.2 M, pH 6.0)-ammonium phosphate buffer (0.2 M, pH 6.0)-water (24:5:5:66, v/v/v/v). Temperature was 35 degrees C, flow rate was 1.5 ml min-1, detection was by UV at 210 nm. Results for a number of commercial samples of various origin are reported.
本文描述了一种用于红霉素液相色谱分析的柱切换技术。该方法能在约1小时内将红霉素A与其所有已知潜在杂质（除次要杂质红霉素D外）分离。该切换技术结合了两根均填充10微米RSil C 18 LL的色谱柱（7.5 cm×4.6 mm和25.0 cm×4.6 mm）。流动相为乙腈 - 硫酸四丁铵（0.2 M，pH 6.0） - 磷酸铵缓冲液（0.2 M，pH 6.0） - 水（24:5:5:66，v/v/v/v）。温度为35℃，流速为1.5 ml min⁻¹，检测波长为210 nm的紫外检测。报告了多个不同来源商业样品的分析结果。
