Stubbs C, Kanfer I
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
J Chromatogr. 1988 May 13;427(1):93-101. doi: 10.1016/0378-4347(88)80107-5.
The simultaneous determination of erythromycin propionate and erythromycin base in serum and urine by high-performance liquid chromatography using oleandomycin as internal standard is described. The separation was achieved on a reversed-phase C18 column employing acetonitrile-0.05 M phosphate buffer (65:35), adjusted to pH 7.0, as the mobile phase with coulometric detection. Hydrolysis of the ester during blood sample collection was minimised by immediate high-speed centrifugation of collected blood samples, followed by separation and immediate freezing of the serum fraction. A solid-phase extraction procedure, combined with a simple phase-separation step was used prior to chromatographic analysis. The method has the necessary precision, sensitivity and accuracy to allow the simultaneous determination of both components in serum and urine following a single 500-mg oral dose of erythromycin estolate.
描述了一种采用柱晶白霉素作为内标，通过高效液相色谱法同时测定血清和尿液中丙酸红霉素和红霉素碱的方法。在反相C18柱上进行分离，采用乙腈-0.05M磷酸盐缓冲液（65:35）（pH值调至7.0）作为流动相，并进行库仑检测。通过对采集的血样立即进行高速离心，随后分离并立即冷冻血清部分，将血样采集过程中酯的水解降至最低。在色谱分析之前，使用了一种固相萃取程序，并结合了一个简单的相分离步骤。该方法具有必要的精密度、灵敏度和准确度，能够在单次口服500mg无味红霉素后同时测定血清和尿液中的两种成分。
