一个悖论：胰岛素抑制抵抗素的表达和分泌，而抵抗素会诱导胰岛素抵抗。
A paradox: insulin inhibits expression and secretion of resistin which induces insulin resistance.
作者信息
Liu Feng, Fan Hong-Qi, Qiu Jie, Wang Bin, Zhang Min, Gu Nan, Zhang Chun-Mei, Fei Li, Pan Xiao-Qing, Guo Mei, Chen Rong-Hua, Guo Xi-Rong
机构信息
Institute of Pediatrics of Nanjing Maternity and Child Health Hospital of the Nanjing Medical University, Nanjing 210004, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2008 Jan 7;14(1):95-100. doi: 10.3748/wjg.14.95.
AIM
To confirm whether insulin regulates resistin expression and secretion during differentiation of 3T3-L1 preadipocytes and the relationship of resistin with insulin resistance both in vivo and in vitro.
METHODS
Supernatant resistin was measured during differentiation of 3T3-L1 preadipocytes. L6 rat myoblasts and hepatoma cell line H4IIE were used to confirm the cellular function of resistin. Diet-induced obese rats were used as an insulin resistance model to study the relationship of resistin with insulin resistance.
RESULTS
Resistin expression and secretion were enhanced during differentiation 3T3-L1 preadipocytes. This cellular differentiation stimulated resistin expression and secretion, but was suppressed by insulin. Resistin also induced insulin resistance in H4IIE hepatocytes and L6 myoblasts. In diet-induced obese rats, serum resistin levels were negatively correlated with insulin sensitivity, but not with serum insulin.
CONCLUSION
Insulin can inhibit resistin expression and secretion in vitro, but insulin is not a major regulator of resistin in vivo. Fat tissue mass affects insulin sensitivity by altering the expression and secretion of resistin.
目的
证实胰岛素在3T3-L1前脂肪细胞分化过程中是否调节抵抗素的表达和分泌，以及抵抗素在体内和体外与胰岛素抵抗的关系。
方法
在3T3-L1前脂肪细胞分化过程中检测上清液中的抵抗素。使用L6大鼠成肌细胞和肝癌细胞系H4IIE来证实抵抗素的细胞功能。以饮食诱导的肥胖大鼠作为胰岛素抵抗模型，研究抵抗素与胰岛素抵抗的关系。
结果
在3T3-L1前脂肪细胞分化过程中，抵抗素的表达和分泌增强。这种细胞分化刺激了抵抗素的表达和分泌，但受到胰岛素的抑制。抵抗素还在H4IIE肝细胞和L6成肌细胞中诱导胰岛素抵抗。在饮食诱导的肥胖大鼠中，血清抵抗素水平与胰岛素敏感性呈负相关，但与血清胰岛素无关。
结论
胰岛素在体外可抑制抵抗素的表达和分泌，但在体内不是抵抗素的主要调节因子。脂肪组织量通过改变抵抗素的表达和分泌来影响胰岛素敏感性。
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