Morohoshi Tomohiro, Kato Masashi, Fukamachi Katsumasa, Kato Norihiro, Ikeda Tsukasa
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, Yoto, Utsunomiya, Japan.
FEMS Microbiol Lett. 2008 Feb;279(1):124-30. doi: 10.1111/j.1574-6968.2007.01016.x.
In tests, Chromobacterium violaceum ATCC 12472 produced several N-acyl-L-homoserine lactones (AHLs). Of these, N-(3-hydroxydecanoyl)-L-homoserine lactone was dominant, and controlled violacein production by quorum sensing. Strain VIR07, an AHL-deficient mutant, did not produce violacein. Violacein production in VIR07 was induced by adding long-chain AHLs (C10-C16), but was inhibited by adding short-chain AHLs (C4-C8). Strain VIR07 showed the response of violacein production when AHLs diffused from a variety of AHL-producing bacteria on agar plates, and thus might be a useful biosensor for recognizing exogenous AHLs.
在测试中，紫色色杆菌ATCC 12472产生了几种N-酰基-L-高丝氨酸内酯（AHLs）。其中，N-（3-羟基癸酰基）-L-高丝氨酸内酯占主导地位，并通过群体感应控制紫菌素的产生。菌株VIR07是一种AHL缺陷型突变体，不产生紫菌素。通过添加长链AHLs（C10-C16）可诱导VIR07产生紫菌素，但添加短链AHLs（C4-C8）则会抑制其产生。当AHLs从琼脂平板上的多种AHL产生菌扩散时，菌株VIR07表现出紫菌素产生的反应，因此可能是一种用于识别外源AHLs的有用生物传感器。
