N-酰基高丝氨酸内酯调节紫色色杆菌模式菌株ATCC 12472中紫菌素的产生。

N-acylhomoserine lactone regulates violacein production in Chromobacterium violaceum type strain ATCC 12472.

作者信息

Morohoshi Tomohiro, Kato Masashi, Fukamachi Katsumasa, Kato Norihiro, Ikeda Tsukasa

机构信息

Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, Yoto, Utsunomiya, Japan.

出版信息

FEMS Microbiol Lett. 2008 Feb;279(1):124-30. doi: 10.1111/j.1574-6968.2007.01016.x.

DOI:10.1111/j.1574-6968.2007.01016.x

PMID:18177311

Abstract

In tests, Chromobacterium violaceum ATCC 12472 produced several N-acyl-L-homoserine lactones (AHLs). Of these, N-(3-hydroxydecanoyl)-L-homoserine lactone was dominant, and controlled violacein production by quorum sensing. Strain VIR07, an AHL-deficient mutant, did not produce violacein. Violacein production in VIR07 was induced by adding long-chain AHLs (C10-C16), but was inhibited by adding short-chain AHLs (C4-C8). Strain VIR07 showed the response of violacein production when AHLs diffused from a variety of AHL-producing bacteria on agar plates, and thus might be a useful biosensor for recognizing exogenous AHLs.

摘要

在测试中，紫色色杆菌ATCC 12472产生了几种N-酰基-L-高丝氨酸内酯（AHLs）。其中，N-（3-羟基癸酰基）-L-高丝氨酸内酯占主导地位，并通过群体感应控制紫菌素的产生。菌株VIR07是一种AHL缺陷型突变体，不产生紫菌素。通过添加长链AHLs（C10-C16）可诱导VIR07产生紫菌素，但添加短链AHLs（C4-C8）则会抑制其产生。当AHLs从琼脂平板上的多种AHL产生菌扩散时，菌株VIR07表现出紫菌素产生的反应，因此可能是一种用于识别外源AHLs的有用生物传感器。