Walter Eric D, Sebby Karl B, Usselman Robert J, Singel David J, Cloninger Mary J
Department of Chemistry and Biochemistry, OpTeC, and Center for Bioinspired Nanomaterials, 108 Gaines Hall, Montana State University, Bozeman, Montana 59717, USA.
J Phys Chem B. 2005 Nov 24;109(46):21532-8. doi: 10.1021/jp0515683.
Starburst dendrimers are receiving considerable attention as templates for the assembly of structured arrays of molecular components. This research motivates the development of improved methods for dendrimer characterization-specifically, for determining the numbers, distributions of numbers, and spatial distribution of molecular species synthetically attached to macromolecular templates. Such information provides the basis for advancing strategies aimed at controlling dendrimer functionalization, and thus represents enabling technology for tailoring the composition and structure of molecular arrays fashioned on dendrimer templates. Moreover, this information is vital to the proper interpretation of ongoing experiments in which dendrimers sparsely functionalized with reporter groups are used as probes. In this article, we report MALDI-TOF mass spectrometry and EPR spectroscopy of heterogeneously functionalized G(4)-PAMAM dendrimers bearing nitroxide spin-labels.
星爆树枝状大分子作为分子组件结构化阵列组装的模板正受到广泛关注。这项研究推动了改进树枝状大分子表征方法的发展，特别是用于确定合成连接到高分子模板上的分子种类的数量、数量分布和空间分布。此类信息为推进旨在控制树枝状大分子功能化的策略提供了基础，因此代表了定制在树枝状大分子模板上构建的分子阵列的组成和结构的使能技术。此外，该信息对于正确解释正在进行的实验至关重要，在这些实验中，用报告基团进行稀疏功能化的树枝状大分子被用作探针。在本文中，我们报告了带有氮氧化物自旋标记的异质功能化G(4)-PAMAM树枝状大分子 的基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和电子顺磁共振波谱（EPR）。
