Gautam Usha Ghimire, Shundo Atsuomi, Gautam Mani Prasad, Takafuji Makoto, Ihara Hirotaka
Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
J Chromatogr A. 2008 May 2;1189(1-2):77-82. doi: 10.1016/j.chroma.2007.12.018. Epub 2007 Dec 15.
In this study, we introduce an attractive stationary phase, poly(4-vinylpyridine)-grafted silica (VP(n)) for normal-phase high-performance liquid chromatography. The retention behavior of polycyclic aromatic hydrocarbons (PAHs) was investigated with VP(n) column under n-hexane/2-propanol mixture as mobile phase. Conventional octadecylated silica, aminopropyl-bonded silica, bare silica and poly(styrene)-grafted silica columns were used as reference columns. Extremely high retention factors were observed for PAHs but not for alkylbenzenes and distinct higher selectivity towards PAHs was observed for the detailed molecular shape such as planarity and aspect ratio. The reason for these results seems to be a multiple interaction effect including an inductive interaction between the pyridyl and aromatic rings.
在本研究中，我们引入了一种用于正相高效液相色谱的极具吸引力的固定相——聚（4-乙烯基吡啶）接枝硅胶（VP(n)）。以正己烷/2-丙醇混合物为流动相，用VP(n)柱研究了多环芳烃（PAHs）的保留行为。使用常规的十八烷基化硅胶柱、氨丙基键合硅胶柱、裸硅胶柱和聚（苯乙烯）接枝硅胶柱作为参比柱。观察到PAHs具有极高的保留因子，而烷基苯则没有，并且对于诸如平面度和长宽比等详细分子形状，观察到对PAHs具有明显更高的选择性。这些结果的原因似乎是包括吡啶基与芳环之间的诱导相互作用在内的多重相互作用效应。
