Shahruzzaman Md, Takafuji Makoto, Ihara Hirotaka
Department of Applied Chemistry and Biochemistry, Kumamoto University, Kurokami, Kumamoto, Japan.
Department of Natural Sciences, Daffodil International University, Dhanmondi, Dhaka, Bangladesh.
J Sep Sci. 2015 Jul;38(14):2403-13. doi: 10.1002/jssc.201500189. Epub 2015 Jun 3.
The amphiphilic polymer-grafted silica was newly prepared as a stationary phase in high-performance liquid chromatography. Poly(4-vinylpyridine) with a trimethoxysilyl group at one end was grafted onto porous silica particles and the pyridyl side chains were quaternized with 1-bromooctadecane. The obtained poly(octadecylpyridinium)-grafted silica was characterized by elemental analysis, diffuse reflectance infrared Fourier transform spectroscopy and Brunauer-Emmett-Teller analysis. The degree of quaternization of the pyridyl groups on the obtained stationary phase was estimated to be 70%. The selective retention behaviors of polycyclic aromatic hydrocarbons including some positional isomers were investigated using poly(octadecylpyridinium)-grafted silica as an amphiphilic polymer stationary phase in high-performance liquid chromatography and results were compared with commercially available polymeric octadecylated silica and phenyl-bonded silica columns. The results indicate that the selectivity toward polycyclic aromatic hydrocarbons exhibited by the amphiphilic polymer stationary phase is higher than the corresponding selectivity exhibited by a conventional phenyl-bonded silica column. However, compared with the polymeric octadecylated silica phase, the new stationary phase presents similar retention behavior for polycyclic aromatic hydrocarbons but different retention behavior particularly for positional isomers of disubstituted benzenes as the aggregation structure of amphiphilic polymers on the surface of silica substrate has been altered during mobile phase variation.
两亲性聚合物接枝硅胶是一种新制备的高效液相色谱固定相。一端带有三甲氧基硅烷基的聚（4-乙烯基吡啶）接枝到多孔硅胶颗粒上，吡啶侧链用1-溴十八烷季铵化。通过元素分析、漫反射红外傅里叶变换光谱和Brunauer-Emmett-Teller分析对所得聚（十八烷基吡啶鎓）接枝硅胶进行了表征。所得固定相上吡啶基团的季铵化程度估计为70%。以聚（十八烷基吡啶鎓）接枝硅胶为两亲性聚合物固定相，在高效液相色谱中研究了包括一些位置异构体在内的多环芳烃的选择性保留行为，并将结果与市售的聚十八烷基化硅胶柱和苯基键合硅胶柱进行了比较。结果表明，两亲性聚合物固定相对多环芳烃的选择性高于传统苯基键合硅胶柱的相应选择性。然而，与聚十八烷基化硅胶相比，新固定相对多环芳烃表现出相似的保留行为，但对二取代苯的位置异构体表现出不同的保留行为，因为在流动相变化过程中，硅胶基质表面两亲性聚合物的聚集结构发生了改变。
