Lefebvre Christophe, Vandenbulcke Franck, Bocquet Béatrice, Tasiemski Aurélie, Desmons Annie, Verstraete Mathilde, Salzet Michel, Cocquerelle Claude
Université de Lille 1, Laboratoire de Neuroimmunologie des Annélides, FRE 2933 Centre National de Recherche Scientifique (CNRS), IFR147, Bâtiment SN3, Université de Lille1, 59655 Villeneuve d'Ascq cedex, France.
Dev Comp Immunol. 2008;32(7):795-807. doi: 10.1016/j.dci.2007.11.007. Epub 2007 Dec 26.
Previous studies evidenced that cystatin B-like gene is specifically expressed and induced in large circulating coelomic cells following bacterial challenge in the leech Theromyzon tessulatum. In order to understand the role of that cysteine proteinase inhibitor during immune response, we investigated the existence of members of cathepsin family. We cloned a cathepsin L-like gene and studied its tissue distribution. Immunohistochemical studies using anti-cathepsin L and anti-cystatin B antibodies and ultrastructural results demonstrated the presence of three distinct coelomic cell populations: (1) the chloragocytes, which were initially defined as large coelomocytes, (2) the granular amoebocytes and (3) small coelomic cells. Among those cells, while chloragocytes contain cystatin B and cathepsin L, granular amoebocytes contain only cathepsin L and the third cell population contains neither cathepsin nor inhibitor. Finally, results evidenced that cathepsin L immunopositive granular amoebocytes are chemoattracted to the site of injury and phagocyte bacteria.
先前的研究表明，在欧洲医蛭受到细菌攻击后，类胱抑素B基因在循环的大型体腔细胞中特异性表达并被诱导。为了了解这种半胱氨酸蛋白酶抑制剂在免疫反应中的作用，我们研究了组织蛋白酶家族成员的存在情况。我们克隆了一个类组织蛋白酶L基因，并研究了其组织分布。使用抗组织蛋白酶L和抗胱抑素B抗体的免疫组织化学研究以及超微结构结果表明存在三种不同的体腔细胞群体：（1）氯细胞，最初被定义为大型体腔细胞；（2）颗粒变形细胞；（3）小型体腔细胞。在这些细胞中，氯细胞含有胱抑素B和组织蛋白酶L，颗粒变形细胞仅含有组织蛋白酶L，而第三种细胞群体既不含有组织蛋白酶也不含有抑制剂。最后，结果表明，组织蛋白酶L免疫阳性的颗粒变形细胞被趋化到损伤部位并吞噬细菌。
