Kim Kyumson, Jang Sung Sik, Kim Sung Ki, Park Jong-Hyun, Heu Sunggi, Ryu Sangryeol
Department of Food and Animal Biotechnology, School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Int J Food Microbiol. 2008 Feb 29;122(1-2):196-203. doi: 10.1016/j.ijfoodmicro.2007.11.072. Epub 2007 Dec 4.
Various food samples in Korea were examined for the presence of Enterobacter sakazakii. Dried shrimp had the highest contamination rate among the examined dried fish products. E. sakazakii isolates were confirmed using an API 20E kit and a polymerase chain reaction (PCR) targeting the rDNA operons. The isolates were subtyped by pulsed-field gel electrophoresis (PFGE) using XbaI to elucidate the genetic diversity of the organisms. Ten pulsotypes were identified using PFGE and 22 types were identified from the random amplified polymorphism DNA (RAPD) assay. An antibiotic resistance test was performed by disk diffusion assay using eight antibiotics: nalidixic acid, ciprofloxacin, chloramphenicol, ampicillin, tetracycline, gentamicin, kanamycin, and cephalothin. Most of the E. sakazakii isolates were resistant to ampicillin or cephalothin but susceptible to the other antibiotics. The analysis of E. sakazakii isolates using PFGE, RAPD, and the antibiotic resistance test identified 18 composite types from 113 isolates, suggesting diverse sources of contamination.
对韩国的各类食品样本进行了阪崎肠杆菌检测。在所检测的鱼干制品中，虾干的污染率最高。使用API 20E试剂盒和针对rDNA操纵子的聚合酶链反应（PCR）对阪崎肠杆菌分离株进行确认。使用XbaI通过脉冲场凝胶电泳（PFGE）对分离株进行亚型分析，以阐明这些微生物的遗传多样性。使用PFGE鉴定出10种脉冲型，从随机扩增多态性DNA（RAPD）分析中鉴定出22种类型。使用8种抗生素通过纸片扩散法进行抗生素耐药性测试：萘啶酸、环丙沙星、氯霉素、氨苄青霉素、四环素、庆大霉素、卡那霉素和头孢噻吩。大多数阪崎肠杆菌分离株对氨苄青霉素或头孢噻吩耐药，但对其他抗生素敏感。使用PFGE、RAPD和抗生素耐药性测试对阪崎肠杆菌分离株进行分析，从113株分离株中鉴定出18种复合类型，表明污染来源多样。
