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坚果、果脯和蜜饯的微生物质量,包括 spp. 的流行情况

Microbiological Quality of Nuts, Dried and Candied Fruits, Including the Prevalence of spp.

作者信息

Berthold-Pluta Anna, Garbowska Monika, Stefańska Ilona, Stasiak-Różańska Lidia, Aleksandrzak-Piekarczyk Tamara, Pluta Antoni

机构信息

Department of Technology and Food Assessment, Division of Milk Technology, Institute of Food Sciences, Warsaw University of Life Sciences-SGGW, 02-787 Warsaw, Poland.

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, 02-787 Warsaw, Poland.

出版信息

Pathogens. 2021 Jul 15;10(7):900. doi: 10.3390/pathogens10070900.

Abstract

genus bacteria are food-borne pathogens. Foods contaminated with spp. may pose a risk to infants or immunocompromised adults. The aim of this study was to determine the microbiological quality of nuts, seeds and dried fruits with special emphasis on the occurrence of spp. Analyses were carried out on 64 samples of commercial nuts (20 samples), dried fruits (24), candied fruits (8), seeds (4), and mixes of seeds, dried fruits and nuts (8). The samples were tested for the total plate count of bacteria (TPC), counts of yeasts and molds, and the occurrence of spp. isolates were identified and differentiated by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragments Length Polymorphism) and RAPD-PCR (Random Amplified Polymorphic DNA by PCR) analysis. TPC, and yeasts and molds were not detected in 0.1 g of 23.4%, 89.1%, and 32.8% of the analyzed samples. In the remaining samples, TPC were in the range of 1.2-5.3 log CFU g. The presence/absence of species was detected in 12 (18.8%) samples of: nuts (10 samples), and mixes (2 samples). The 12 strains of spp. included: (3 strains), (5), and (4). The results of this study contribute to the determination of the presence and species identification of spp. in products of plant origin intended for direct consumption.

摘要

属细菌是食源性病原体。被该属细菌污染的食品可能会对婴儿或免疫功能低下的成年人构成风险。本研究的目的是确定坚果、种子和干果的微生物质量,特别关注该属细菌的存在情况。对64份市售坚果(20份样本)、干果(24份)、蜜饯(8份)、种子(4份)以及种子、干果和坚果混合物(8份)样本进行了分析。对样本进行了细菌总数(TPC)、酵母菌和霉菌计数以及该属细菌存在情况的检测。通过PCR-RFLP(聚合酶链反应-限制性片段长度多态性)和RAPD-PCR(PCR随机扩增多态性DNA)分析对分离株进行鉴定和区分。在23.4%、89.1%和32.8%的分析样本中,0.1 g样本未检测到TPC、酵母菌和霉菌。在其余样本中,TPC范围为1.2-5.3 log CFU/g。在12份(18.8%)样本中检测到该属细菌的存在/不存在,这些样本包括:坚果(10份样本)和混合物(2份样本)。这12株该属细菌包括:(3株)、(5株)和(4株)。本研究结果有助于确定直接食用的植物源性产品中该属细菌的存在情况和种类鉴定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effa/8308658/081054ede12a/pathogens-10-00900-g001.jpg

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