Yusuf Tony E, Matthes Kai, Brugge William R
GI Unit, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Gastrointest Endosc. 2008 May;67(6):957-61. doi: 10.1016/j.gie.2007.08.020. Epub 2008 Feb 21.
EUS-guided photodynamic therapy (PDT) with the photosensitizing agent porfimer sodium has been shown to be effective in ablation of pancreatic tissue.
The objective of this study was to determine the effectiveness and safety of EUS PDT with verteporfin, a photosensitizer associated with less photosensitivity.
Prospective investigation of 6 swine that received an IV injection of 6 mg/m2 of verteporfin before EUS.
Hospital animal laboratory.
The tail of the pancreas was located with EUS and was used to guide the placement of a light catheter. The pancreatic tail was exposed to 10, 15, or 20 minutes of laser light (689 nm).
Follow-up abdominal CT, liver and renal function tests, and serum pancreatic enzymes levels were performed. Histology of the pancreas was obtained 7 days after the procedure on necropsy.
Localized tissue necrosis within the pancreatic tail (range 6.6-30.5 mm in diameter) was seen in all animals. The diameter of the necrotic tissue was directly related to the dose of light. No post-procedural complications were observed.
Normal animal model (lack of malignant tissue).
EUS-guided PDT of porcine pancreas with verteporfin achieved localized pancreatic tissue ablation in a dose-related fashion.
已证明使用光敏剂卟吩姆钠的超声内镜引导下光动力疗法（PDT）对胰腺组织消融有效。
本研究的目的是确定使用维替泊芬（一种光敏性较低的光敏剂）进行超声内镜引导下光动力疗法的有效性和安全性。
对6头猪进行前瞻性研究，这些猪在超声内镜检查前静脉注射6 mg/m²的维替泊芬。
医院动物实验室。
通过超声内镜定位胰腺尾部，并用于引导光导管的放置。胰腺尾部接受10、15或20分钟的激光照射（689 nm）。
进行随访腹部CT、肝肾功能检查和血清胰酶水平检测。术后7天在尸检时获取胰腺组织学样本。
所有动物的胰腺尾部均出现局限性组织坏死（直径范围6.6 - 30.5 mm）。坏死组织的直径与光照剂量直接相关。未观察到术后并发症。
正常动物模型（缺乏恶性组织）。
使用维替泊芬进行超声内镜引导下猪胰腺光动力疗法以剂量相关方式实现了局限性胰腺组织消融。
