Leite Marina Concli, Galland Fabiana, Brolese Giovana, Guerra Maria Cristina, Bortolotto Josiane Woutheres, Freitas Rodrigo, Almeida Lucia Maria Vieira de, Gottfried Carmem, Gonçalves Carlos-Alberto
Programa de Pós-Graduação em Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
J Neurosci Methods. 2008 Mar 30;169(1):93-9. doi: 10.1016/j.jneumeth.2007.11.021. Epub 2007 Dec 4.
S100B expression, particularly extracellular S100B, is used as a parameter of glial activation and/or death in several situations of brain injury. Several immunoassays for S100B measurement are available, which differ with regard to specificity, sensitivity, sample application, and, of course, economic costs. We standardized two protocols for S100B measurement (range between 1.9pg and 10ng/mL) in human and rat samples from brain and adipose tissues, blood serum, cerebrospinal fluid, urine and cell culture. Abundance and secretion of this protein in adipose tissue reinforces the caution about its origin in blood serum. Interestingly, S100B recognition was affected by the redox status of the protein. This aspect should be considered in S100B measurement, assuming that oxidized and reduced forms possibly coexist in vivo and the equilibrium can be modified by oxidative stress of physiological or pathological conditions or even by obtaining sample conditions.
在多种脑损伤情况下，S100B表达，尤其是细胞外S100B，被用作神经胶质激活和/或死亡的一个参数。有几种用于测量S100B的免疫测定方法，它们在特异性、灵敏度、样品应用方面存在差异，当然，经济成本也不同。我们对来自人脑和大鼠脑及脂肪组织、血清、脑脊液、尿液和细胞培养物的样品中S100B测量（范围在1.9皮克至10纳克/毫升之间）的两种方案进行了标准化。该蛋白在脂肪组织中的丰度和分泌情况，强化了对其血清来源的谨慎态度。有趣的是，S100B的识别受到该蛋白氧化还原状态的影响。在S100B测量中应考虑这一方面，假定氧化型和还原型可能在体内共存，并且这种平衡可能会因生理或病理条件下的氧化应激甚至样品获取条件而改变。
