金黄色葡萄球菌的SarA与sarA启动子结合以调控基因表达。
SarA of Staphylococcus aureus binds to the sarA promoter to regulate gene expression.
作者信息
Cheung Ambrose L, Nishina Koren, Manna Adhar C
机构信息
Department of Microbiology and Immunology, Vail 210, Dartmouth Medical School, Hanover, NH 03766, USA.
出版信息
J Bacteriol. 2008 Mar;190(6):2239-43. doi: 10.1128/JB.01826-07. Epub 2008 Jan 4.
Abstract
The 375-bp sarA open reading frame is driven by three promoters, P1, P3, and P2. Using gel shift and DNase I footprinting assays, we found that SarA binds to two 26-bp sequences and one 31-bp sequence within the P1 and P3 promoters, respectively. Together with the results of transcription analyses, our data indicate that SarA binds to its own promoter to down-regulate sarA expression.
摘要
375碱基对的sarA开放阅读框由三个启动子P1、P3和P2驱动。通过凝胶迁移和DNase I足迹分析，我们发现SarA分别与P1和P3启动子内的两个26碱基对序列和一个31碱基对序列结合。结合转录分析结果，我们的数据表明SarA与其自身启动子结合以下调sarA表达。
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