Liao C-H
Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, PA 19038, USA.
Lett Appl Microbiol. 2008 Feb;46(2):232-6. doi: 10.1111/j.1472-765X.2007.02302.x. Epub 2007 Dec 20.
To investigate the growth of salmonellae on sprouting alfalfa seeds as affected by the inoculum size, microbial load and Pseudomonas fluorescens 2-79.
Alfalfa seeds pre-inoculated with < or =10(1)-10(3) CFU g(-1) of salmonellae and with or without Ps. fluorescens 2-79 were sprouted in glass jars and the population of salmonellae were determined daily for up to 6 days. The population of salmonellae on germinating seeds reached the maximum 2-3 days after sprouting when total bacterial count reached the maximum (10(9) CFU g(-1)). The population of salmonellae on sprouting seeds not treated with Ps. fluorescens 2-79 showed a net increase of 3-4 log units. However, the population of salmonellae on alfalfa seeds treated with Ps. fluorescens 2-79 showed a net increase of only 1-2 log units. Disinfection of seeds with calcium hypochlorite enhanced the growth of salmonellae.
Treatment of seeds with Ps. fluorescens 2-79 reduced the growth of salmonellae by 2-3 log units.
The potential of Ps. fluorescens 2-79 as a biological agent for use in control of salmonellae on sprouting seeds was demonstrated and warrants further investigation.
研究接种量、微生物负荷及荧光假单胞菌2-79对发芽苜蓿种子上沙门氏菌生长的影响。
将预先接种了≤10(1)-10(3) CFU g(-1)沙门氏菌且添加或未添加荧光假单胞菌2-79的苜蓿种子在玻璃瓶中发芽，每天测定沙门氏菌数量，持续6天。发芽种子上的沙门氏菌数量在发芽后2-3天达到最大值，此时总细菌数达到最大值(10(9) CFU g(-1))。未用荧光假单胞菌2-79处理的发芽种子上的沙门氏菌数量净增加3-4个对数单位。然而，用荧光假单胞菌2-79处理的苜蓿种子上的沙门氏菌数量净增加仅1-2个对数单位。用次氯酸钙对种子进行消毒可促进沙门氏菌生长。
用荧光假单胞菌2-79处理种子可使沙门氏菌生长减少2-3个对数单位。
证明了荧光假单胞菌2-79作为一种生物制剂用于控制发芽种子上沙门氏菌的潜力，值得进一步研究。
