Sun Wei-Wei, Liu Jia, Wang Xu-Yang, Zhang Lian-Shuang, Zhang Wei, Li Li-Yan, Li Hui, Wang Ting-Hua
Institute of Neurological Disease, West China Hospital, Sichuan University, Chengdu 610041 [corrected] China.
Neurosci Lett. 2008 Jan 31;431(2):112-7. doi: 10.1016/j.neulet.2007.11.061. Epub 2007 Dec 8.
Changes in the platelet derived growth factor (PDGF) in the spared dorsal root ganglia (DRG) and associated spinal dorsal horns were evaluated in cats subjected to unilateral removal of L1-L5 and L7-S2 DRG, sparing the L6 DRG. The number of PDGF immunopositive neurons and protein expression decreased significantly in the spared DRG and associated dorsal horns of the L3 and L6 cord segments at 3 days post-operation (dpo). It bottomed to the lowest level at 7 dpo in the DRG, then returned to the control level at 14 dpo; while in the L6 dorsal horn, it rapidly increased at 7 dpo and exceeded the control level at 14 dpo. This showed a significant upregulation in the spared DRG and associated spinal dorsal horns, especially in the L6 cord segment following a transient decrease. Meanwhile, a significant upregulation of PDGF mRNA was also seen in L6 DRG and L3 and L6 dorsal horns at 3 dpo. The upregulation of the endogenous PDGF in the said structures indicated a potential role of this factor in spinal cord plasticity after partial dorsal root ganglia removal in cats.
在单侧切除L1-L5和L7-S2背根神经节（DRG）而保留L6 DRG的猫中，评估了保留的背根神经节和相关脊髓背角中血小板衍生生长因子（PDGF）的变化。术后3天（dpo），保留的DRG以及L3和L6脊髓节段相关背角中PDGF免疫阳性神经元的数量和蛋白表达显著降低。DRG中在7 dpo时降至最低水平，然后在14 dpo时恢复到对照水平；而在L6背角中，其在7 dpo时迅速增加，并在14 dpo时超过对照水平。这表明在短暂降低后，保留的DRG和相关脊髓背角中出现了显著上调，尤其是在L6脊髓节段。同时，在3 dpo时，L6 DRG以及L3和L6背角中也观察到PDGF mRNA的显著上调。上述结构中内源性PDGF的上调表明该因子在猫部分背根神经节切除后脊髓可塑性中具有潜在作用。
