[猫部分背根切断术后脊髓和保留的背根神经节中Sema3A和NP-1的表达]

[Expression of Sema3A and NP-1 in spinal cord and spared dorsal root ganglion after partial dorsal root rhizotomy of cat].

作者信息

Ding Yan, Zhang Wei, Huang Qian, Peng Jin, Zhao Xiu-jun, Xu Ai-li, Zhou Xue

机构信息

Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Jan;39(1):48-51.

PMID:18390198

Abstract

OBJECTIVE

To investigate the expression of semaphorin 3A (Sema3A) and neuropilin 1 (NP-1) in spinal cord and dorsal root ganglion after partial dorsal root rhizotomy.

METHODS

15 adult cats were used for this study and divided into 3 groups: normal control group, 7 d and 14 d postoperative groups (7Th day and 14th day groups) undergoing unilateral partial dorsal root rhizotomy. The L3, L5 and L6 segments of spinal cord and L6 dorsal root ganglia (DRG) in operated side were made into frozen sections. By immunohistochemistry ABC method, the sections of spinal cord were stained with specific Sema3A antibody, and L6 DRG were stained with NP-1 antibody. The mean optical density (OD) of Sema3A immunoreactivity in dorsal horn was measured and the number of NP-1 positive medium-small sized neurons in spared DRG was counted.

RESULTS

After partial dorsal root rhizotomy, in L3 segment the expression of Sema3A decreased in 7th day group (0.25 +/- 0. 14) compared with that in normal group(0. 37 +/- 0.87) (P < 0.05), but kept the level along to 14th day group (0.27 +/- 0.09); in L5 segment, the expression of Sema3A decrea sed in 7th day group (0.26 +/- 0.11) (P < 0.05), and then recovered to normal level in 14th day group (0.33 +/- 0.09); in L6 segment, OD values in dorsal horn had no changes to all groups. The number of NP-1 positive medium-small sized neurons in spared DRG (30.85 +/- 10.26) was decreased in 7th day group (P < 0.05), compared with that in normal group (45.06 +/- 12.47), while increased in 14th day group (P < 0.05).

CONCLUSION

Changes in the expression of Sema3A in spinal cord and the expression of NP-1 in L6 DRG after partial root rhizotomy may be involved in collateral sprouting of spared root in superficial lamina.

摘要

目的

探讨部分背根切断术后脊髓和背根神经节中信号素3A（Sema3A）和神经纤毛蛋白1（NP-1）的表达情况。

方法

本研究选用15只成年猫，分为3组：正常对照组、单侧部分背根切断术后7天组和14天组（7天组和14天组）。将手术侧脊髓的L3、L5和L6节段以及L6背根神经节（DRG）制成冰冻切片。采用免疫组织化学ABC法，脊髓切片用特异性Sema3A抗体染色，L6 DRG用NP-1抗体染色。测量背角Sema3A免疫反应性的平均光密度（OD），并计数保留DRG中NP-1阳性中小神经元的数量。

结果

部分背根切断术后，在L3节段，7天组Sema3A的表达（0.25±0.14）较正常组（0.37±0.87）降低（P<0.05），但至14天组维持该水平（0.27±0.09）；在L5节段，7天组Sema3A的表达降低（0.26±0.11）（P<0.05），14天组恢复至正常水平（0.33±0.09）；在L6节段，背角的OD值在所有组中均无变化。保留DRG中NP-1阳性中小神经元的数量（30.85±10.26）在7天组较正常组（45.06±12.47）减少（P<0.05），而在14天组增加（P<0.05）。