Bloemhof H, Van Dijk K N, De Graaf S S, Vendrig D E, Uges D R
Department of Pharmacy, University Hospital, Groingen, Netherlands.
J Chromatogr. 1991 Dec 6;572(1-2):171-9. doi: 10.1016/0378-4347(91)80481-q.
A column-switching high-performance liquid chromatographic method was developed for the determination of vincristine in serum. Sample preparation was carried out by means of on-line column-extraction, using a C18 reversed-phase preconcentration column. This technique is simple (minimizing manual sampling errors), rapid (reduction of time and costs) and can be easily automated. Both ultraviolet and electrochemical detection are possible, but the latter shows a cleaner chromatogram and is, by the use of a new electrochemical detector, far more sensitive (detection limit 0.3 microgram/l at a signal-to-noise ratio of 3). A matrix study was carried out (using human serum and urine and two kinds of calf's serum). Although it appeared that the system was matrix-dependent, no difference in matrix effects could be found in the serum or plasma of different patients. Controls for human serum analysis should be prepared in human serum. With the method described, pharmacokinetic studies of vincristine in children can be performed.
建立了一种柱切换高效液相色谱法用于测定血清中的长春新碱。样品前处理通过在线柱萃取进行，使用C18反相预浓缩柱。该技术简单（将人工采样误差降至最低）、快速（减少时间和成本）且易于自动化。紫外检测和电化学检测均可，但后者的色谱图更干净，并且通过使用新型电化学检测器，灵敏度更高（在信噪比为3时检测限为0.3微克/升）。进行了基质研究（使用人血清、尿液和两种小牛血清）。尽管该系统似乎依赖于基质，但在不同患者的血清或血浆中未发现基质效应的差异。用于人血清分析的对照品应用人血清制备。采用所述方法可对儿童长春新碱进行药代动力学研究。
