Vendrig D E, Holthuis J J, Erdélyi-Tóth V, Hulshoff A
J Chromatogr. 1987 Feb 20;414(1):91-100. doi: 10.1016/0378-4347(87)80027-0.
A sensitive and selective high-performance liquid chromatographic (HPLC) method for the determination of vinblastine and vincristine in plasma and urine is described. The drugs are isolated from 1.0 ml of the biological fluid with a solid-phase extraction column (Bond-Elut Diol). The HPLC method was combined with electrochemical detection at +850 mV versus an Ag/AgCl reference electrode. The detection limit is 100 pg for vinblastine and 250 pg for vincristine with a signal-to-noise ratio of 3, which permits the determination of these compounds in biological fluids at the nanogram level. Evaluation of the isolation method revealed that the drug recoveries and the reproducibility of the extraction procedure depend on the batch number of the solid-phase extraction column used.
描述了一种灵敏且具选择性的高效液相色谱（HPLC）方法，用于测定血浆和尿液中的长春碱和长春新碱。通过固相萃取柱（Bond-Elut二醇柱）从1.0 ml生物流体中分离出这些药物。该HPLC方法与相对于Ag/AgCl参比电极在+850 mV下的电化学检测相结合。长春碱的检测限为100 pg，长春新碱的检测限为250 pg，信噪比为3，这使得能够在纳克水平测定生物流体中的这些化合物。对分离方法的评估表明，药物回收率和萃取程序的重现性取决于所用固相萃取柱的批次编号。
