离子交换色谱法、等电沉淀法和反相高效液相色谱法用于分离单个心肌肌球蛋白轻链的比较。

Comparison of ion-exchange chromatography, isoelectric precipitation and reversed-phase high-performance liquid chromatography for the separation of individual cardiac myosin light chains.

作者信息

Shi Q W, Olley P M, Jackowski G

机构信息

Max Bell Research Center, Toronto General Hospital, Department of Clinical Biochemistry, University of Toronto, Canada.

出版信息

J Chromatogr. 1991 Dec 6;572(1-2):59-71. doi: 10.1016/0378-4347(91)80473-p.

DOI:10.1016/0378-4347(91)80473-p

PMID:1818076

Abstract

Three modified procedures for the separation of cardiac myosin light chains are carefully compared. Ion-exchange chromatography gives a purified cardiac myosin light chain 1, whereas light chain 2 is always contaminated by light chain 1. Reversed-phase high-performance liquid chromatography gives the best resolution of these light chains and needs only 20 min for each run. However, it requires pure preparation of myosin light chains before separation. Isoelectric precipitation is the simplest procedure and suitable for large quantities of material. Although it gives the highest yield the separation is not adequate. A modified and rapid procedure for the isolation of cardiac and skeletal total myosin light chains is also presented.

摘要

对三种改良的心肌肌球蛋白轻链分离方法进行了仔细比较。离子交换色谱法可得到纯化的心肌肌球蛋白轻链1，而轻链2总是被轻链1污染。反相高效液相色谱法对这些轻链的分离效果最佳，每次运行仅需20分钟。然而，在分离之前需要肌球蛋白轻链的纯制剂。等电沉淀是最简单的方法，适用于大量材料。虽然它的产量最高，但分离效果并不理想。还介绍了一种改良的快速分离心肌和骨骼肌总肌球蛋白轻链的方法。