Hiratsuka T
Biochim Biophys Acta. 1980 Oct 21;625(2):369-73. doi: 10.1016/0005-2795(80)90301-3.
A simple and efficient procedure is presented for the separation of pig cardiac myosin light chains. The method employs only three repetitive isoelectric precipitations without column chromatography, and is suitable for use with large quantities of material. The method also permits the recovery of homogeneous light chains with good yields.
本文介绍了一种简单有效的猪心肌肌球蛋白轻链分离方法。该方法仅采用三次重复等电沉淀,无需柱色谱法,适用于大量材料。该方法还能以高回收率获得均一的轻链。