Fain J N, Sacks H S, Buehrer B, Bahouth S W, Garrett E, Wolf R Y, Carter R A, Tichansky D S, Madan A K
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Int J Obes (Lond). 2008 May;32(5):810-5. doi: 10.1038/sj.ijo.0803790. Epub 2008 Jan 8.
The purpose of this study was to determine the relative distribution of omentin and visfatin mRNA in human epicardial, peri-internal mammary, upper thoracic, upper abdominal and leg vein subcutaneous adipose tissue as well as the distribution of omentin in the nonfat cells and adipocytes of human omental adipose tissue.
Omentin is found in human omentum but not subcutaneous fat. Omentin and visfatin are considered markers of visceral abdominal fat.
The mRNA content of omentin and visfatin was measured by qRT-PCR analysis of fat samples removed from humans undergoing cardiac or bariatric surgery.
Omentin mRNA in internal mammary fat was 3.5%, that in the upper thoracic subcutaneous fat was 4.7% while that in the other subcutaneous fat depots was less than 1% of omentin in epicardial fat. The distribution of visfatin mRNA did not vary between the five depots. Omentin mRNA was preferentially expressed in the nonfat cells of omental adipose tissue since the omentin mRNA content of isolated adipocytes was 9% of that in nonfat cells, and similar results were seen for visfatin. The amount of omentin mRNA in differentiated adipocytes was 0.3% and that of visfatin 4% of that in nonfat cells. The amount of omentin mRNA in preadipocytes was virtually undetectable while that of visfatin was 3% of that in freshly isolated nonfat cells from omental adipose tissue.
Omentin mRNA is predominantly found in epicardial and omental human fat whereas visfatin mRNA is found to the same extent in epicardial, subcutaneous and omental fat.
本研究旨在确定网膜素和内脂素mRNA在人的心外膜、胸廓内、上胸部、上腹部及腿部静脉皮下脂肪组织中的相对分布情况，以及网膜素在人网膜脂肪组织的非脂肪细胞和脂肪细胞中的分布。
网膜素存在于人网膜中而非皮下脂肪中。网膜素和内脂素被认为是腹部内脏脂肪的标志物。
通过对接受心脏手术或减肥手术的患者所取脂肪样本进行qRT-PCR分析，测定网膜素和内脂素的mRNA含量。
胸廓内脂肪中的网膜素mRNA含量为心外膜脂肪中网膜素的3.5%，上胸部皮下脂肪中的为4.7%，而其他皮下脂肪库中的则不到1%。五个脂肪库中内脂素mRNA的分布没有差异。网膜素mRNA优先在网膜脂肪组织的非脂肪细胞中表达，因为分离出的脂肪细胞中网膜素mRNA含量是非脂肪细胞中的9%，内脂素也有类似结果。分化脂肪细胞中网膜素mRNA的含量为非脂肪细胞中的0.3%，内脂素为4%。前脂肪细胞中网膜素mRNA几乎检测不到，而内脂素为网膜脂肪组织新鲜分离的非脂肪细胞中的3%。
网膜素mRNA主要存在于人的心外膜和网膜脂肪中，而内脂素mRNA在心外膜、皮下和网膜脂肪中的含量相同。
