Kar Sanjay, Mandal Asish, Mohapatra Pradeep K Das, Samanta Saptadip, Pati Bikash R, Mondal Keshab C
Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
J Ind Microbiol Biotechnol. 2008 Apr;35(4):245-9. doi: 10.1007/s10295-007-0292-7. Epub 2008 Jan 8.
In the present study, the optimum conditions for the production of xylanase by immobilized spores of Trichoderma reesei SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200, 300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase significantly with increasing concentration of alginate and reached a maximum yield of 3.12+/-0.18 U ml(-1) at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth cycle (3.36+/-0.2 U ml(-1)).
在本研究中，确定了里氏木霉SAF3固定化孢子在海藻酸钙珠粒中生产木聚糖酶的最佳条件。还研究了半连续发酵过程中珠粒在木聚糖酶生产期间的操作稳定性。考虑了海藻酸钠浓度（1%、2%、3%和4%）和初始细胞负载量（每瓶100、200、300、400和500个珠粒）对木聚糖酶生产的影响。发现随着海藻酸钠浓度的增加，木聚糖酶的产量显著增加，在2%（w/v）时达到最高产量3.12±0.18 U ml-1。固定化细胞可持续生产木聚糖酶达10个循环，并在第4个循环达到最高水平（3.36±0.2 U ml-1）。
