Halim Yuniwaty, Devianita Devianita, Hardoko Hardoko, Handayani Ratna, Soedirga Lucia C
Food Technology Department, Universitas Pelita Harapan, Jl. M.H. Thamrin Boulevard, Lippo Karawaci, Tangerang 15811, Indonesia.
Faculty of Fisheries and Marine Sciences, Brawijaya University, Jl. Veteran No. 1, Malang 65113, Indonesia.
Food Technol Biotechnol. 2022 Mar;60(1):4-10. doi: 10.17113/ftb.60.01.22.6994.
Shrimp shells contain chitin that can be further processed into acetylglucosamine, which has been extensively used to treat joint damage. has a strong chitinolytic activity and may be utilized in the form of immobilized cells in repeated fermentation. Pumice is a porous and rigid stone that offers superior mechanical strength, making it suitable for immobilization.
In the research submerged fermentation with different pumice stone sizes and pumice stone/growth medium ratios (/) was carried out for 4 days at 37 °C and pH=7.0. The optimum pumice stone size and pumice stone/growth medium ratio (/) were used to determine the optimum fermentation cycle for the production of acetylglucosamine using immobilized .
Pumice stones of 1.0 cm×1.0 cm×1.0 cm and pumice stone/growth medium ratio of 1:5 were found to be the optimum conditions for successful immobilization of (90.0±1.6) % cells and production of (331.4±7.3) g/L acetylglucosamine. The highest acetylglucosamine concentration of (323.0±2.5) g/L was obtained in the first fermentation cycle, which then decreased and remained stable throughout the last three cycles.
, a strong chitinolytic bacterium previously isolated from rotten shrimp shells, was used for the first time in immobilized form to produce acetylglucosamine. The findings in this research showed the potential use of cells immobilized in pumice stone for continuous production of acetylglucosamine in repeated fermentation.
虾壳含有几丁质，可进一步加工成乙酰葡糖胺，已被广泛用于治疗关节损伤。[细菌名称]具有很强的几丁质分解活性，可制成固定化细胞用于重复发酵。浮石是一种多孔坚硬的石头，具有优异的机械强度，适合用于固定化。
在本研究中，使用不同尺寸的浮石和不同的浮石/生长培养基比例（/），于37℃、pH = 7.0的条件下进行4天的深层发酵。采用最佳的浮石尺寸和浮石/生长培养基比例（/）来确定使用固定化[细菌名称]生产乙酰葡糖胺的最佳发酵周期。
发现1.0 cm×1.0 cm×1.0 cm的浮石和1:5的浮石/生长培养基比例是成功固定（90.0±1.6）%的[细菌名称]细胞并生产（331.4±7.3）g/L乙酰葡糖胺的最佳条件。在第一个发酵周期中获得了最高乙酰葡糖胺浓度（323.0±2.5）g/L，随后浓度下降，并在最后三个周期中保持稳定。
[细菌名称]是一种先前从腐烂虾壳中分离出的强大几丁质分解细菌，首次以固定化形式用于生产乙酰葡糖胺。本研究结果表明，固定在浮石中的[细菌名称]细胞在重复发酵中具有连续生产乙酰葡糖胺的潜在用途。
